Parallel and flexible fluorescent imaging using two-photon RESOLFT super-resolution microscopy with spatial light modulator control

High resolution imaging in three dimension is important for biological research. The RESOLFT (Reversible Saturable Optical Fluorescence Transitions) fluorescent microscopy is one technique which can achieve lateral super-resolution imaging. Two-photon microscopy naturally generate high resolution in the longitudinal direction with less background compared to single photon excitation. We combine these two methods to realize three-dimensional high-resolution imaging. This super-resolution method also is limited in imaging speed. We use a spatial light modulator (SLM) as a flexible phase mask of the microscopy. It is used to compensate the system aberration, as well as increasing the imaging speed. The parallel scanning generates multiple super-resolution focuses as an array or in arbitrary positions by phase retrieval calculation. This microscopy combined with SLM control could applied to high throughput 3D imaging or multiple spots tracking in high-resolution.

Department dc:contributor.department

Massachusetts Institute of Technology. Department of Mechanical Engineering.

Grantor dc:publisher

Massachusetts Institute of Technology

Year dc:date.issued

2015

Author dc:creator

• Xue, Yi, S.M. Massachusetts Institute of Technology

Advisor dc:contributor.advisor

• Peter T. C. So.

• Mechanical Engineering.

Statement dc:rights

• M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission.

Licence dc:rights.uri

• http://dspace.mit.edu/handle/1721.1/7582

Language dc:language.iso

eng

Handle dc:identifier.uri

http://hdl.handle.net/1721.1/100346

OAI identifier oai:identifier

oai:dspace.mit.edu:1721.1/100346