Measurement of Internal Movements of the Escherichia Coli Ribosome Using Forster Resonance Energy Transfer and Microsecond, Continuous -Flow Turbulent Mixing in Micro-Fabricated Devices

The second half of this thesis is independent of the above. We present the design, construction and implementation of micro-fabricated, continuous-flow, turbulent mixing devices that can mix two or three fluids to complete homogeneity on the molecular scale in the microsecond range. The prototypical designs are compact, portable, simple to fabricate and consume smaller sample volumes than current technology. We characterize the turbulent mixing process in microfluidic channels with fluorescence intensity and lifetime imaging and show that both the dependence of mixing times and pressure drop on the flow velocity and Reynolds number agree well with theoretical expectations for turbulent pipe flow. The novelties in this work are: the new methods of fabrication which enable production of three-dimensional geometries in glass with micro-fabrication methods, the implementation of turbulent mixing and measurement at microsecond timescales and showing the ability to generalize theoretical expectations for turbulence in pipes with micrometer scale dimensions.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Physics

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2015

Author dc:creator

• Majumdar, Zigurts Krishna

Contributors dc:contributor

• Clegg, Robert M.

• Biology, Molecular

Language dc:language

eng

Identifier

(MiAaPQ)AAI3202138

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/80528