Chip -Based Detection of Protein Cancer Markers

A similar approach was then employed to directly detect protein targets. Instead of DNA, monoclonal antibodies were patterned in the gap. The detector was then exposed to the protein target and gold nanoparticles functionalized with polyclonal antibodies. The target protein was used to immobilize the nanoparticle probes and silver staining was once again employed to bridge the gap It was determined that this technique can be used to detect protein targets both in buffer and serum samples with a 100 pM sensitivity limit.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Bioengineering

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2015

Author dc:creator

• Goluch, Edgar D.

Contributors dc:contributor

• Chang Liu

• Biology, Molecular

Language dc:language

eng

Identifier

(MiAaPQ)AAI3301266

OAI record oai:identifier

https://www.ideals.illinois.edu/oai-pmh?verb=GetRecord&metadataPrefix=etdms&identifier=oai%3Awww.ideals.illinois.edu%3A2142%2F79577