Particle-enhanced biomolecule detection using silicon photonic microring resonators

Protein detection is of great importance in both research and clinical settings. The use of proteins as diagnostic markers of disease is a driving force behind the development of advanced protein detection techniques. Of particular interest is the development of a detection platform which is both highly sensitive and capable of simultaneously quantitating a number of targets, as a single biomarker is seldom capable of providing the information necessary to make sophisticated diagnostic determinations. The Bailey Group has developed a detection platform based on silicon photonic microring resonators which is capable of making sensitive measurements of proteins, as well as other biomolecules, in complex matrices. The following chapters present the work I have completed towards the development of particle-based detection schemes for use on the microring resonator platform. Chapter 2 details the initial attempts at detection of a particle-like species, namely Bean pod mottle virus, from the complex matrix of minimally-processed leaf extracts. Chapter 3 details the initial developments of magnetic-bead based assays for lower limits of detection and extended dynamic range. Chapter 4 details the extension of previous magnetic particle detection by incorporating a permanent magnet into the chip holder in order to direct magnetic beads towards the surface of the sensor, effectively shortening analysis times and further decreasing limits of detection. Chapter 5 begins to move away from bead-based assays and discusses studies directly comparing bead-based signal enhancement strategies with enzyme-based strategies. Chapter 6 discusses the initial developments into a multiplexed biomarker panel for the detection and discrimination of liver diseases. Chapter 7 speculates on the future of the methods I have developed and discusses where I believe there is room for improvement and further development arising from my work. Finally, there are two appendices which present small studies and initial work which may be a valuable reference for future students.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Chemistry

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2015

Author dc:creator

• McClellan, Melinda

Contributors dc:contributor

• Bailey, Ryan C.
• Murphy, Catherine J.
• Scheeline, Alexander
• Schroeder, Charles M.

• Particle-enhanced
• Biomolecule Detection
• Microring Resonators
• Biosensors

Statement dc:rights

• Copyright 2015 Melinda McClellan

Handle dc:identifier

http://hdl.handle.net/2142/78293

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/78293