{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/78293"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/78293","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Particle-enhanced biomolecule detection using silicon photonic microring resonators","abstract":"Protein detection is of great importance in both research and clinical settings. The use of proteins as diagnostic markers of disease is a driving force behind the development of advanced protein detection techniques. Of particular interest is the development of a detection platform which is both highly sensitive and capable of simultaneously quantitating a number of targets, as a single biomarker is seldom capable of providing the information necessary to make sophisticated diagnostic determinations. The Bailey Group has developed a detection platform based on silicon photonic microring resonators which is capable of making sensitive measurements of proteins, as well as other biomolecules, in complex matrices. The following chapters present the work I have completed towards the development of particle-based detection schemes for use on the microring resonator platform. Chapter 2 details the initial attempts at detection of a particle-like species, namely Bean pod mottle virus, from the complex matrix of minimally-processed leaf extracts. Chapter 3 details the initial developments of magnetic-bead based assays for lower limits of detection and extended dynamic range. Chapter 4 details the extension of previous magnetic particle detection by incorporating a permanent magnet into the chip holder in order to direct magnetic beads towards the surface of the sensor, effectively shortening analysis times and further decreasing limits of detection. Chapter 5 begins to move away from bead-based assays and discusses studies directly comparing bead-based signal enhancement strategies with enzyme-based strategies. Chapter 6 discusses the initial developments into a multiplexed biomarker panel for the detection and discrimination of liver diseases. Chapter 7 speculates on the future of the methods I have developed and discusses where I believe there is room for improvement and further development arising from my work. Finally, there are two appendices which present small studies and initial work which may be a valuable reference for future students.","abstract_html":"Protein detection is of great importance in both research and clinical settings. The use of proteins as diagnostic markers of disease is a driving force behind the development of advanced protein detection techniques. Of particular interest is the development of a detection platform which is both highly sensitive and capable of simultaneously quantitating a number of targets, as a single biomarker is seldom capable of providing the information necessary to make sophisticated diagnostic determinations. The Bailey Group has developed a detection platform based on silicon photonic microring resonators which is capable of making sensitive measurements of proteins, as well as other biomolecules, in complex matrices. The following chapters present the work I have completed towards the development of particle-based detection schemes for use on the microring resonator platform. Chapter 2 details the initial attempts at detection of a particle-like species, namely Bean pod mottle virus, from the complex matrix of minimally-processed leaf extracts. Chapter 3 details the initial developments of magnetic-bead based assays for lower limits of detection and extended dynamic range. Chapter 4 details the extension of previous magnetic particle detection by incorporating a permanent magnet into the chip holder in order to direct magnetic beads towards the surface of the sensor, effectively shortening analysis times and further decreasing limits of detection. Chapter 5 begins to move away from bead-based assays and discusses studies directly comparing bead-based signal enhancement strategies with enzyme-based strategies. Chapter 6 discusses the initial developments into a multiplexed biomarker panel for the detection and discrimination of liver diseases. Chapter 7 speculates on the future of the methods I have developed and discusses where I believe there is room for improvement and further development arising from my work. Finally, there are two appendices which present small studies and initial work which may be a valuable reference for future students.","abstract_has_math":false,"creators":["McClellan, Melinda"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Chemistry","degree_department":null,"school":null,"contributors":["Bailey, Ryan C.","Murphy, Catherine J.","Scheeline, Alexander","Schroeder, Charles M."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2015,"date_issued":"2015-07-22T22:15:34Z","date_published":"2015-07-22T22:15:34Z","updated_at":"2026-07-22T22:26:11Z","subjects":["Particle-enhanced","Biomolecule Detection","Microring Resonators","Biosensors"],"languages":[],"rights":["Copyright 2015 Melinda McClellan"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/2142/78293","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Bailey, Ryan C.","Murphy, Catherine J.","Scheeline, Alexander","Schroeder, Charles M."]},{"key":"dc:creator","label":"Author","values":["McClellan, Melinda"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2015-07-22T22:15:34Z","2015-05","2015-01-15","2015-5"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Chemistry"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Particle-enhanced","Biomolecule Detection","Microring Resonators","Biosensors"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2015 Melinda McClellan"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/78293"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Protein detection is of great importance in both research and clinical settings. The use of proteins as diagnostic markers of disease is a driving force behind the development of advanced protein detection techniques. Of particular interest is the development of a detection platform which is both highly sensitive and capable of simultaneously quantitating a number of targets, as a single biomarker is seldom capable of providing the information necessary to make sophisticated diagnostic determinations. The Bailey Group has developed a detection platform based on silicon photonic microring resonators which is capable of making sensitive measurements of proteins, as well as other biomolecules, in complex matrices. The following chapters present the work I have completed towards the development of particle-based detection schemes for use on the microring resonator platform. Chapter 2 details the initial attempts at detection of a particle-like species, namely Bean pod mottle virus, from the complex matrix of minimally-processed leaf extracts. Chapter 3 details the initial developments of magnetic-bead based assays for lower limits of detection and extended dynamic range. Chapter 4 details the extension of previous magnetic particle detection by incorporating a permanent magnet into the chip holder in order to direct magnetic beads towards the surface of the sensor, effectively shortening analysis times and further decreasing limits of detection. Chapter 5 begins to move away from bead-based assays and discusses studies directly comparing bead-based signal enhancement strategies with enzyme-based strategies. Chapter 6 discusses the initial developments into a multiplexed biomarker panel for the detection and discrimination of liver diseases. Chapter 7 speculates on the future of the methods I have developed and discusses where I believe there is room for improvement and further development arising from my work. Finally, there are two appendices which present small studies and initial work which may be a valuable reference for future students.","Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2015-07-22 without embargo terms","The student, Melinda McClellan, accepted the attached license on 2015-01-12 at 14:42.","The student, Melinda McClellan, submitted this Dissertation for approval on 2015-01-12 at 14:51.","This Dissertation was approved for publication on 2015-01-15 at 10:58.","DSpace SAF Submission Ingestion Package generated from Vireo submission #7677 on 2015-07-22 at 10:29:35","Made available in DSpace on 2015-07-22T22:15:34Z (GMT). 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Of particular interest is the development of a detection platform which is both highly sensitive and capable of simultaneously quantitating a number of targets, as a single biomarker is seldom capable of providing the information necessary to make sophisticated diagnostic determinations. The Bailey Group has developed a detection platform based on silicon photonic microring resonators which is capable of making sensitive measurements of proteins, as well as other biomolecules, in complex matrices. The following chapters present the work I have completed towards the development of particle-based detection schemes for use on the microring resonator platform. Chapter 2 details the initial attempts at detection of a particle-like species, namely Bean pod mottle virus, from the complex matrix of minimally-processed leaf extracts. Chapter 3 details the initial developments of magnetic-bead based assays for lower limits of detection and extended dynamic range. Chapter 4 details the extension of previous magnetic particle detection by incorporating a permanent magnet into the chip holder in order to direct magnetic beads towards the surface of the sensor, effectively shortening analysis times and further decreasing limits of detection. Chapter 5 begins to move away from bead-based assays and discusses studies directly comparing bead-based signal enhancement strategies with enzyme-based strategies. Chapter 6 discusses the initial developments into a multiplexed biomarker panel for the detection and discrimination of liver diseases. Chapter 7 speculates on the future of the methods I have developed and discusses where I believe there is room for improvement and further development arising from my work. Finally, there are two appendices which present small studies and initial work which may be a valuable reference for future students.","Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2015-07-22 without embargo terms","The student, Melinda McClellan, accepted the attached license on 2015-01-12 at 14:42.","The student, Melinda McClellan, submitted this Dissertation for approval on 2015-01-12 at 14:51.","This Dissertation was approved for publication on 2015-01-15 at 10:58.","DSpace SAF Submission Ingestion Package generated from Vireo submission #7677 on 2015-07-22 at 10:29:35","Made available in DSpace on 2015-07-22T22:15:34Z (GMT). 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