Analysis of the Structure and Cell-Specific Transcription of the Bovine Parathyroid Hormone Gene (Micrococcal Nuclease)

Two EcoR I restriction fragments containing PTH gene sequences were isolated from a partial bovine library prepared using EcoR I cleaved high molecular weight bovine DNA and lambda phage charon 30 as a cloning vector. Restriction and sequence analyses of the two fragments indicate that they are identical. This finding supports the conclusion from genomic Southern blot data that there is one copy of the PTH gene per haploid genome. The complete sequence of the bovine PTH gene has been determined and compared to the sequence published for human and rat PTH genes. Remarkable sequence features of the bovine gene include: the high AT content (68%), including very long stretches of alternating AT, the exon-functional domain correspondence, and the presence of two TATA sequences in the 5' flanking region of the gene. Primer extension and S1 mapping of the 5' termini of PTH mRNA indicate that both TATA boxes function in directing transcription in vivo. In addition, both TATA boxes appear to direct transcription in vitro. However, the relative levels of transcriptional initiation from the two TATA boxes were different in vitro as compared to in vivo.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Molecular and Integrative Physiology

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2015

Author dc:creator

• Weaver, Christine Alston

• Biology, Molecular

Language dc:language

eng

Identifier

(UMI)AAI8701646

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/77683