Studies on the Efficiency of Energy Conservation in the Initial Stages of Photophosphorylation

The first section of the thesis is an investigation of the accessibility of the thylakoid lumen to exogenous buffers, and the effects of added buffers on presteady state phosphorylation. Using the method of silicon oil centrifugation to measure uptake of radioactively labelled compounds, we estimated permeability coefficients for sorbitol, sucrose, and Tris, Tricine, and MOPS buffers and found these compounds to be only slowly permeant. However, incubation of thylakoids in Tricine for one hour allowed enough buffer to be taken up to generate a predictable lag in the onset of ATP synthesis. When membranes so incubated were illuminated by a train of single turnover flashes, ATP synthesis began long before the added Tricine could have been titrated.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Botany

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2015

Author dc:creator

• Flores, Susan Cecille

• Biology, Plant Physiology

Language dc:language

eng

Identifier

(UMI)AAI8409921

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/77654