Studies on the Efficiency of Energy Conservation in the Initial Stages of Photophosphorylation
type: CreativeWork
description: The first section of the thesis is an investigation of the accessibility of the thylakoid lumen to exogenous buffers, and the effects of added buffers on presteady state phosphorylation. Using the method of silicon oil centrifugation to measure uptake of radioactively labelled compounds, we estimated permeability coefficients for sorbitol, sucrose, and Tris, Tricine, and MOPS buffers and found these compounds to be only slowly permeant. However, incubation of thylakoids in Tricine for one hour allowed enough buffer to be taken up to generate a predictable lag in the onset of ATP synthesis. When membranes so incubated were illuminated by a train of single turnover flashes, ATP synthesis began long before the added Tricine could have been titrated.
abstract: The first section of the thesis is an investigation of the accessibility of the thylakoid lumen to exogenous buffers, and the effects of added buffers on presteady state phosphorylation. Using the method of silicon oil centrifugation to measure uptake of radioactively labelled compounds, we estimated permeability coefficients for sorbitol, sucrose, and Tris, Tricine, and MOPS buffers and found these compounds to be only slowly permeant. However, incubation of thylakoids in Tricine for one hour allowed enough buffer to be taken up to generate a predictable lag in the onset of ATP synthesis. When membranes so incubated were illuminated by a train of single turnover flashes, ATP synthesis began long before the added Tricine could have been titrated.
author: Flores, Susan Cecille
language: eng
keywords: Biology, Plant Physiology
date published: 2015-05-14T15:19:14Z
date modified: 2026-07-22T22:26:11Z
provider: University of Illinois - Urbana-Champaign
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: University of Illinois at Urbana-Champaign

PropertyValue
property ID: Handle
value: http://hdl.handle.net/2142/77654