Structure and Assembly of the Photosynthetic Membrane of Rhodobacter Sphaeroides

The structure of the photosynthetic reaction center of Rhodobacter sphaeroides was studied using chemical cross-linking reagents. Four intra-complex cross-linked polypeptide complexes were produced when purified reaction centers, reaction centers incorporated into liposomes, or intact chromatophore membranes were cross-linked. The complexes were composed of H-M, H-L, H-M-L, or L-M. The cross-linking of cytochrome c to soluble or membrane bound reaction centers was observed using any of the four cross-linking reagents. The products were a cytochrome c linked to either the M or L polypeptide. The results indicated L and M must be exposed from the membrane surface near a binding site for cytochrome c and all three subunits must be in close proximity.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Biology

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2015

Author dc:creator

• Hoger, Jeffrey Harlen

• Biology, General

Language dc:language

eng

Identifier

(UMI)AAI8701510

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/77645