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Showing 1 to 20 of 63 for “"Rhodobacter sphaeroides"”.
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Molecular Genetic Analysis of Rhodobacter Sphaeroides Light-Harvesting Complexes
The genes for the Rhodobacter sphaeroides light-harvesting, B875-$\beta$ and B875-$\alpha$ polypeptides (pufB and pufA) are closely linked to the genes for the reaction center-L and reaction center-M polypeptides (pufL and pufM) on what has been termed the puf operon (gene order pufB,A,L,M). The …
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Studies of a DNA modification methyltransferase from Rhodobacter sphaeroides
… RsrI methyltransferase (M$\cdot$RsrI) from Rhodobacter sphaeroides recognizes duplex d(GAATTC) and deposits methyl groups at the $N\sp6$ position of central adenine. M$\cdot$RsrI was purified to homogeneity from R. sphaeroides, and its gene cloned and sequenced. The purification used four …
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Coordination of Carbon Dioxide and Nitrogen Metabolism in Rhodobacter sphaeroides
… pathways of the nonsulfur purple bacterium <i>Rhodobacter sphaeroides</i> were studied simultaneously <i>in vivo</i> to determine their influence upon each other. <i>Rb. sphaeroides</i> is capable of nitrogen fixation via nitrogenase catalysis and carbon dioxide fixation via the …
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Structure and Assembly of the Photosynthetic Membrane of Rhodobacter Sphaeroides
… of the photosynthetic reaction center of Rhodobacter sphaeroides was studied using chemical cross-linking reagents. Four intra-complex cross-linked polypeptide complexes were produced when purified reaction centers, reaction centers incorporated into liposomes, or intact chromatophore …
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Studies of Cytochrome C Oxidases From Rhodobacter Sphaeroides and Vibrio Cholerae
… incorporation of heme c into cbb3 enzymes from Rhodobacter sphaeroides and Vibrio cholerae. Results also show that every heme c is involved in the electron translocations in a consequential way and is important for the activity and stability of the enzyme complex. Sequence alignment and …
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Structure-Function Relationship Studies of the Rhodobacter Sphaeroides Cytochrome AA(3)
… can be used to produce ATP by ATP-Synthase. Rhodobacter sphaeroides cytochrome c oxidase, the focus of this thesis, serves as an excellent model to systematically understand the more complicated mammalian cytochrome c oxidases. The role of a putative proton channel identified in the X-ray …
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The Study of Aa3-Type Cytochrome C Oxidase in Rhodobacter Sphaeroides
Cytochrome c oxidase is the final electron acceptor in the respiratory chain and catalyzes the highly exergonic oxygen reduction reaction to water and forms a transmembrane electrochemical proton gradient. This transmembrane gradient is used by ATP synthase to produce ATP. The oxygen chemistry …
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Identification and characterization of the ribosomal RNA operons from Rhodobacter sphaeroides
… present in the facultative photoheterotroph Rhodobacter sphaeroides were identified, cloned and sequenced. DNA sequence analysis has identified the 16S, 23S and 5S rRNAs, two tRNAs (ile and ala) in the 16S-23S spacer region, and an f-met tRNA immediately following the 5S rRNa gene of all …
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Mutational studies of the cytochrome bc(1) complex of Rhodobacter sphaeroides
The cytochrome $bc\sb1$ complex is a multisubunit integral membrane protein. This enzyme is a central component in electron transport where it oxidizes quinol while forming a transmembrane proton gradient. The cytochrome b subunit is the locus of two distinct quinol binding sites as well as the …
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Studies of cytochrome c oxidases from Rhodobacter sphaeroides and Vibrio cholerae
… incorporation of heme c into cbb3 enzymes from Rhodobacter sphaeroides and Vibrio cholerae. Results also show that every heme c is involved in the electron translocations in a consequential way and is important for the activity and stability of the enzyme complex. Sequence alignment and …
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The study of aa3-type cytochrome c oxidase in Rhodobacter sphaeroides
Cytochrome c oxidase is the final electron acceptor in the respiratory chain and catalyzes the highly exergonic oxygen reduction reaction to water and forms a transmembrane electrochemical proton gradient. This transmembrane gradient is used by ATP synthase to produce ATP. The oxygen chemistry …
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Cytochrome C Oxidation by the Bacterial Photosynthetic Reaction Center From Rhodobacter Sphaeroides
… of the photosynthetic reaction center (RC) from Rhodobacter sphaeroides and soluble c-type cytochromes has been studied by redox potentiometry and kinetic absorbance spectroscopy. Under intense continuous illumination, in the presence of excess reactants, the RC turnover rate was seen to depend …
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Purification and Characterization of the Cytochrome BC(1) Complex From Rhodobacter Sphaeroides
… $bc\sb1$ complex) has been obtained from Rhodobacter sphaeroides. The enzyme was extracted from chromatophores using dodecyl maltoside in the presence of glycerol, and was purified by anion exchange and gel filtration chromatography. SDS-polyacrylamide gel electrophoresis showed the …
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Molecular genetic studies on the cytochrome bc(1) complex of Rhodobacter sphaeroides
Restriction data tranferred 2014-07-01T11:24:23-05:00 Original Data Group with Access UIUC Users [automated] Release Date: none Reason: ETDs are only available to UIUC Users without author permission
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Molecular genetic analysis of puf andpuc operon expression in Rhodobacter sphaeroides 2.4.1
The deletion of the puf intercistronic terminator resulted in both the loss of the smallest 0.5-kilobase (kb) puf transcript in addition to an approximate 10-fold increase in transcriptional read-through of the mutated region to more distal pufL gene, suggesting that the proximal intercistronic …
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Biochemically realistic MD and kinetic models of the Rhodobacter sphaeroides bc1 complex
… membrane for a more natural model of the Rb. sphaeroides bc1 complex which in past studies has lacked several details in composition of the fatty acids and relative quantities of each lipid. Past studies have shown some distortion on MD relaxation relating to a large void volume in the protein …
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Heterogeneity of the Bc1 Complex Subunits and Qo Site Occupants From Rhodobacter Sphaeroides
… Trials to crystallize bc1 complex from R. sphaeroides, a membrane-bound four-subunit respiratory protein that shares physical and catalytic properties with the vertebrate bc1 complex, have been undertaken and results help understand the problems of heterogeneity and solutions that can …
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Biochemical Studies on the Catalytic Cycle of Cytochrome C Oxidase in Rhodobacter Sphaeroides
Cytochrome c oxidase from Rhodobacter sphaeroides exhibits properties similar to those of mitochondrial bovine oxidase. The X-ray crystal structure of cytochrome c oxidase from Paracoccus denitrificans reveals two proton channels leading to the binuclear center. (Iwata, S., Ostermeier, C., Ludwig, …
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Identification and regulation of genes involved in carbon dioxide fixation in Rhodobacter sphaeroides
… the genome of the photoheterotrophic bacterium, Rhodobacter sphaeroides, was shown to contain several genes involved in CO$\sb2$ fixation, namely fructose 1,6-bisphosphatase (fbpA), phosphoribulokinase (prkA), a 37-kDa polypeptide (designed CFX A) with a putative regulatory role (cfxA), and Form …
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