Production of Zein From Dry Milled Corn by Solvent Extraction and Ultrafiltration

This research focused on developing a process for the production of low cost zein from corn using a combination of solvent extraction and membrane technology. Extraction of zein from dry milled whole corn was optimum with 70% ethanol at 50°C using a solvent-to-solids ratio of 6 ml ethanol per g corn for 45 minutes. Zein concentration in extract was 3--9 g/l. Continuous column extraction was also best at 50°C and 70% ethanol; a solvent ratio of 1 ml/g resulted in zein concentrations of 17 g/l in the extract. Multiple extractions of the same corn with fresh ethanol resulted in a yield of 85% after 4 extractions, while multiple extractions of fresh corn with the same ethanol resulted in high zein concentration in the extract (15 g/l). The zein extracted in our research was comparable in amino acid analysis and SDS-PAGE profiles to commercial samples.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Food Science

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2015

Author dc:creator

• Shukla, Rishi

Contributors dc:contributor

• Cheryan, Munir

• Agriculture, Food Science and Technology
• Engineering, Agricultural
• Engineering, Chemical
• Engineering, Packaging

Language dc:language

eng

Identifier

(UMI)AAI9971193

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/77467