{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/77467"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/77467","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Production of Zein From Dry Milled Corn by Solvent Extraction and Ultrafiltration","abstract":"This research focused on developing a process for the production of low cost zein from corn using a combination of solvent extraction and membrane technology. Extraction of zein from dry milled whole corn was optimum with 70% ethanol at 50°C using a solvent-to-solids ratio of 6 ml ethanol per g corn for 45 minutes. Zein concentration in extract was 3--9 g/l. Continuous column extraction was also best at 50°C and 70% ethanol; a solvent ratio of 1 ml/g resulted in zein concentrations of 17 g/l in the extract. Multiple extractions of the same corn with fresh ethanol resulted in a yield of 85% after 4 extractions, while multiple extractions of fresh corn with the same ethanol resulted in high zein concentration in the extract (15 g/l). The zein extracted in our research was comparable in amino acid analysis and SDS-PAGE profiles to commercial samples.","abstract_html":"This research focused on developing a process for the production of low cost zein from corn using a combination of solvent extraction and membrane technology. Extraction of zein from dry milled whole corn was optimum with 70% ethanol at 50°C using a solvent-to-solids ratio of 6 ml ethanol per g corn for 45 minutes. Zein concentration in extract was 3--9 g/l. Continuous column extraction was also best at 50°C and 70% ethanol; a solvent ratio of 1 ml/g resulted in zein concentrations of 17 g/l in the extract. Multiple extractions of the same corn with fresh ethanol resulted in a yield of 85% after 4 extractions, while multiple extractions of fresh corn with the same ethanol resulted in high zein concentration in the extract (15 g/l). The zein extracted in our research was comparable in amino acid analysis and SDS-PAGE profiles to commercial samples.","abstract_has_math":false,"creators":["Shukla, Rishi"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Food Science","degree_department":null,"school":null,"contributors":["Cheryan, Munir"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2015,"date_issued":"2015-05-13T15:44:26Z","date_published":"2015-05-13T15:44:26Z","updated_at":"2026-07-22T22:26:10Z","subjects":["Agriculture, Food Science and Technology","Engineering, Agricultural","Engineering, Chemical","Engineering, Packaging"],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["(UMI)AAI9971193"],"render_values":[{"text":"(UMI)AAI9971193","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/77467","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Cheryan, Munir"]},{"key":"dc:creator","label":"Author","values":["Shukla, Rishi"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2015-05-13T15:44:26Z","10000-01-01","2000"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Food Science"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Agriculture, Food Science and Technology","Engineering, Agricultural","Engineering, Chemical","Engineering, Packaging"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/77467","(UMI)AAI9971193"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["This research focused on developing a process for the production of low cost zein from corn using a combination of solvent extraction and membrane technology. Extraction of zein from dry milled whole corn was optimum with 70% ethanol at 50°C using a solvent-to-solids ratio of 6 ml ethanol per g corn for 45 minutes. Zein concentration in extract was 3--9 g/l. Continuous column extraction was also best at 50°C and 70% ethanol; a solvent ratio of 1 ml/g resulted in zein concentrations of 17 g/l in the extract. Multiple extractions of the same corn with fresh ethanol resulted in a yield of 85% after 4 extractions, while multiple extractions of fresh corn with the same ethanol resulted in high zein concentration in the extract (15 g/l). The zein extracted in our research was comparable in amino acid analysis and SDS-PAGE profiles to commercial samples.","Several commercially available polymeric and inorganic membranes were evaluated for stability, flux and rejection in aqueous ethanol solutions. Regenerated cellulose, surface-modified/cross-linked composite and ceramic membranes had better stability than polysulfone or cellulose acetate membranes.","Three polymeric membranes and one inorganic membrane were selected for further studies with model zein solutions in a cross-flow cell. Flux with the Osmonics-Membrex MX25 was 45 liters per square meter per hour (LMH) at 5 g/l zein and 25 LMH at 150 g/l zein at 20 psi and 50°C. Zein rejections were 88% and the Cg value was 340 g/l. The Membralox 200 A ceramic membrane gave a flux of 70 LMH at 5 g/l zein at 15 psi and 50°C, with a zein rejection of 80%. Pilot scale trials done with real extracts confirmed model solution data.","The process developed here can be easily integrated into any corn processing facility, especially dry-grind ethanol plants. The solvent extraction step involves only mixing and a filtration/centrifugation step. The filtered extract, after further treatment if necessary to improve protein purity, can be concentrated using solvent stable membranes to at least 250 g/l, which results in a recycle of over 97% of the extraction solvent, thus substantially lowering energy requirements.","Made available in DSpace on 2015-05-13T15:44:26Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 9971193.PDF: 5858939 bytes, checksum: aa0c8bced530e4945d6696a45c28d6f6 (MD5) Previous issue date: 2000","Embargo set by: Seth Robbins for item 78678 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","153 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2000."]},{"key":"dc:title","label":"Title","values":["Production of Zein From Dry Milled Corn by Solvent Extraction and Ultrafiltration"]}]}],"canonical_facts":{"dc:contributor":["Cheryan, Munir"],"dc:creator":["Shukla, Rishi"],"dc:date":["2015-05-13T15:44:26Z","10000-01-01","2000"],"dc:description":["This research focused on developing a process for the production of low cost zein from corn using a combination of solvent extraction and membrane technology. Extraction of zein from dry milled whole corn was optimum with 70% ethanol at 50°C using a solvent-to-solids ratio of 6 ml ethanol per g corn for 45 minutes. Zein concentration in extract was 3--9 g/l. Continuous column extraction was also best at 50°C and 70% ethanol; a solvent ratio of 1 ml/g resulted in zein concentrations of 17 g/l in the extract. Multiple extractions of the same corn with fresh ethanol resulted in a yield of 85% after 4 extractions, while multiple extractions of fresh corn with the same ethanol resulted in high zein concentration in the extract (15 g/l). The zein extracted in our research was comparable in amino acid analysis and SDS-PAGE profiles to commercial samples.","Several commercially available polymeric and inorganic membranes were evaluated for stability, flux and rejection in aqueous ethanol solutions. Regenerated cellulose, surface-modified/cross-linked composite and ceramic membranes had better stability than polysulfone or cellulose acetate membranes.","Three polymeric membranes and one inorganic membrane were selected for further studies with model zein solutions in a cross-flow cell. Flux with the Osmonics-Membrex MX25 was 45 liters per square meter per hour (LMH) at 5 g/l zein and 25 LMH at 150 g/l zein at 20 psi and 50°C. Zein rejections were 88% and the Cg value was 340 g/l. The Membralox 200 A ceramic membrane gave a flux of 70 LMH at 5 g/l zein at 15 psi and 50°C, with a zein rejection of 80%. Pilot scale trials done with real extracts confirmed model solution data.","The process developed here can be easily integrated into any corn processing facility, especially dry-grind ethanol plants. The solvent extraction step involves only mixing and a filtration/centrifugation step. The filtered extract, after further treatment if necessary to improve protein purity, can be concentrated using solvent stable membranes to at least 250 g/l, which results in a recycle of over 97% of the extraction solvent, thus substantially lowering energy requirements.","Made available in DSpace on 2015-05-13T15:44:26Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 9971193.PDF: 5858939 bytes, checksum: aa0c8bced530e4945d6696a45c28d6f6 (MD5) Previous issue date: 2000","Embargo set by: Seth Robbins for item 78678 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","153 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2000."],"dc:identifier":["http://hdl.handle.net/2142/77467","(UMI)AAI9971193"],"dc:language":["eng"],"dc:subject":["Agriculture, Food Science and Technology","Engineering, Agricultural","Engineering, Chemical","Engineering, Packaging"],"dc:title":["Production of Zein From Dry Milled Corn by Solvent Extraction and Ultrafiltration"],"dc:type":["text"],"thesis:degree_discipline":["Food Science"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:26:10Z"}