Influence of Dietary Protein Intake on the Molecular Aspects of 7,12-Dimethylbenz(a)anthracene Induced Mammary Carcinogenesis (Cancer, Liver)

The influence of prior protein intake in rats on the metabolism and DNA-binding of 7,12-dimethylbenz(a)anthracene (DMBA) by their isolated mammary epithelial cell aggregates was examined. DMBA was metabolized to bay-region anti- and syn- dihydrodiol-epoxides that bound to deoxyadenosine and deoxyguanosine of mammary cell DNA. During the initial 24 hours of incubation of the mammary cells, production of the syn- stereoisomer increased in a linear fashion. The production of the anti- dihydrodiol-epoxide increased rapidly following a 6-hour delay and was inhibited by actinomycin D. Rats were fed diets containing 7.5 or 15% protein supplied as casein. Restricting the protein intake of rats resulted in a decrease in metabolism and binding of DMBA to DNA in the isolated mammary cells after 6 hours of incubation. The syn- dihydrodiol-epoxide DMBA:deoxyadenosine adduct predominated in mammary cells from rats fed either concentration of protein. The quantity of extracellular water-soluble metabolites amounted to only 14% of total metabolism at 6 hours.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Food Science

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2015

Author dc:creator

• Singletary, Keith William

• Health Sciences, Nutrition

Language dc:language

eng

Identifier

(UMI)AAI8623411

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/77455