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Showing 1 to 20 of 44 for “"actinomycin D"”.
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The Effects of Actinomycin D and Acth on Circadian Steroidogenesis of Hamster Adrenals
Rhythmicity is predominant in nature. Sunrise is followed by sunset in a highly predictable periodic sequence. Living organisms are known to be influenced by such periodically occurring phenomena. De Mairan, as early as 1729, observed leaf movements of plants and found these variations to be …
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Some in vivo effects of triiodothyronine on purine nucleotide synthesis
… was observed in the GMP. Treatment of rats with actinomycin D prior to hormone injection did not prevent the stimulation of synthesis due to triiodothyronine. Actinomycin D alone stimulated the incorporation of the precursor into the soluble nucleotides and magnified the effects of the hormone in …
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Regulation and function of interphase histone H1 phosphorylation in pluripotent cell differentiation
… genes. Moreover, inhibiting transcription with actinomycin D induces the accumulation of pS187-H1.4 at promoters and gene bodies. Notably, the levels of pS187-H1.4 enrichment after actinomycin D treatment or cell differentiation reflect the extent of CDK9 recruitment at the same loci. …
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mRNA stability in soma and neurites of cultured neuronal cells
… cells following inhibition of transcription with actinomycin-D. Less stable transcripts were enriched for GU-rich elements in their 3' UTRs. Around 12% of alternative splicing isoform pairs differed in stability, and cassette alternative ("skipped") exons that negatively impact stability are …
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Utilization of Poly(A)-Binding Protein in Monitoring the Formation of mRNP Granules in Toxoplasma gondii
… localized in the cytoplasm under treatment with actinomycin D. Second, we also found that TgPABP aggregates into microscopically visible granules, as an indicative of mRNPs. Upon salubrinal treatment, mRNPs were formed in both extracellular and in intracellular parasites whereas cycloheximide can …
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Influence of Dietary Protein Intake on the Molecular Aspects of 7,12-Dimethylbenz(a)anthracene Induced Mammary Carcinogenesis (Cancer, Liver)
… following a 6-hour delay and was inhibited by actinomycin D. Rats were fed diets containing 7.5 or 15% protein supplied as casein. Restricting the protein intake of rats resulted in a decrease in metabolism and binding of DMBA to DNA in the isolated mammary cells after 6 hours of incubation. …
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Interrelationships among estrogen, insulin-like growth factor-I and cyclic adenosine monophosphate in the regulation of uterine progesterone and estrogen receptors
… inhibitors, or by pre-treatment of cells with actinomycin D or cycloheximide. These results suggest that regulation of PR occurs at the level of the PR gene, and that induction of PR stimulated by these agents requires activation of estrogen receptor (ER) and the action of protein kinases. …
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Translation and transcription are required for endogenous bursting after long term removal of neuromodulators
… (CHX) and the transcription inhibitor Actinomycin D (ACD). The results suggest that early transcription and late protein synthesis play an important role in the recovery process of the rhythmic activity in the pyloric network of the crab STG. Late protein synthesis probably involves ion …
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The effect of thyroid hormones an growth and metabolism of two species of teleost fish
… and RNA in liver and muscle of tilapia and carp. Actinomycin D inhibited this response to a certain extent. Thyroid hormones seem to be more important in the early than in later life of the tilapia as far as the growth is concerned. Temperature mediates the effects of T4 and T3 on cellular level …
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Development and characterization of cell-free amino acid incorporation systems from <u>Cellvibrio gilvus</u>
… but not effected significantly by DNAase and actinomycin D. The optimum pH and temperature for ¹⁴C-leucine incorporation were 7.8 and 300° C, respectively. Polyamiaes (putrescine, spermine, and spermidine) had a slight stimulatory effect on poly U-directed.¹⁴C-phenylalanine incorporation, but …
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Methods to Detect Apoptosis in Equine Peripheral Blood Neutrophils from Normal Healthy Adult Horses
… presence of four known inducers of apoptosis; actinomycin D, staurosporin, cycloheximide and sodium hypochlorite, at four different concentrations (table 2). A fifth population was to induce necrosis using a freeze-thaw cycle and bleach. A control sample was examined (no inducer) to determine …
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Genomic and non genomic effects of elevated concentration of anabolic steroids in human neuronal cells
… 40% in nandrolonetreated cells. Treatment with actinomycin D (10-5M), a transcription inhibitor, revealed that nandrolone may regulate MOPr mRNA stability. In SH-SY5Y cells transfected with a human MOPr luciferase promoter/reporter construct, nandrolone did not alter the rate of gene …
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Untersuchungen zur Apoptose durch das Kontakthapten NiCl 2 in humanen Nabelschnurendothelzellen
… Cycloheximid sowie des Transkritionsinhibitors Actinomycin D zeigte sich eine dosisabhängige Zunahme der Apotposerate, die offenbar eine Inhibition proteinsyntheseabhängiger und somit vor Apoptose schützender Mechanismen erfordert. Die Synthese dieser zytoprotektiven Proteine ist von einer …
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Cytoplasmic RNA-dependent RNA-synthesis in maturing chicken erythrocytes.
… intact cells in the presence of [³H]-uridine and actinomycin D was shown to be very similar with respect to S-value and the ratio of UMP/uridine incorporated. This similarity suggests the presence of a ribosome bound RNA-dependent RNA polymerase activity in intact cells.
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Nova Regulation of Alternative Splicing in the CNS
… foci are disrupted by treatment the cells with actinomycin-D, consistent with the hypothesis that the formation andmaintenance of Nova foci is an active process that is transcription dependent. Finally, differential gene expression screens were undertaken, leading to the identification number of …
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An in vitro system for studying the mechanism of action of thyroid hormones
… effect on nuclear RNA synthesis. DNase and actinomycin D inhibited nuclear RNA synthesis induced by a high ATP/GTP ratio. Based on these results, it was proposed that thyroid hormones regulate the synthesis of AMP and GMP from IMP and maintain a high adenine nucleotide/ guanine nucleotide …
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The study of wheat striate mosaic virus (WSMV) in wheat callus tissue culture
… by autoradiography. By applying RNA inhibitors (actinomycin D, 100 μg/ml and 5-Fluorouracil, 100 μg/ml) and protein inhibitors (cycloheximide, 100 μg/ml and puromycin, 200 μg/ml), viral RNA and protein synthesis were studied. Assuming that there are no changes in specific activities of the …
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Activation of HGF/Met Signaling Induces Delayed STAT3 Phosphorylation
… blocked by pretreatment with cycloheximide and actinomycin D. Interestingly, the conditioned media treated with NGF for 2 hour induced phosphorylation of STAT3 just within 15 miniute when applied to the new culture of NIH3T3 cells. Thus, we supposed that a newly synthesized product was released …
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Cytochrome P450 mRNA profile in human breast cancer cell lines.
… cell lines - using the RNA synthesis inhibitor actinomycin D, followed by the agonists - showed a significant reduction (p < 0.05) of CYP4Z1 mRNA levels. It also inhibited CYP4Z1 induction by either progesterone, dexamethasone or pirinixic acid, indicating that these agonists have effects on …
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