Tetracycline-Associated Regulation of the Transfer Genes of the Bacteroides Conjugative Transposon CTnDOT

Many human colonic Bacteroides spp. harbor a conjugative transposon (CTn) called CTnDOT. CTnDOT carries two antibiotic resistance genes tetQ and ermF. A distinctive feature of CTnDOT is that both its excision and transfer are stimulated by tetracycline. The regulation of excision has been described previously. I provide here the first characterization of the organization and regulation of the CTnDOT transfer (tra) genes. RT-PCR analysis of the region containing the tra genes showed that these genes are transcriptionally coupled in an operon and that expression of this operon is regulated at the transcriptional level. The transcription initiation site of the operon was located 290 bp upstream of the start codon of the first tra gene, traA.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Microbiology

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2014

Author dc:creator

• Jeters, Robert Thomas

Contributors dc:contributor

• Salyers, Abigail A.

• Biology, Microbiology

Identifier

(UMI)AAI3392080

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/72516