Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 153 for “"PCR analysis"”.
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Quantitative Real-Time PCR Analysis of Colchicine Pathway and Rhizome Developmental Genes
… real-time polymerase chain reaction (qRT-PCR), a comparative analysis of gene expression in G. superba may reveal differential gene expression of selected candidate genes. Reference genes were selected and analyzed for normalization of qRT-PCR data. The most stable reference gene, UBC22a, …
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Molecular techniques for studying <i>Fusarium</i> ear blight of wheat
This work has compared polymerase chain reaction (PCR) assays and traditional visual disease assessment to evaluate the severity of <i>Fusarium</i> ear blight (FEB) ofwheat under field and glasshouse conditions. In a field trial, PCR analysis highlighted the problem of diagnosis of FEB of wheat …
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A study assessing forage quality and desert bighorn sheep (Ovis canadensis nelsoni) diet in central Nevada using microhistology and molecular analysis
… to forage quality data. Microhistological analysis and PCR analysis determined the frequency of forage types in desert bighorn sheep fecal samples collected throughout the Clan Alpines. Forage types found in fecal samples were compared seasonally and diversity indices were compared to sites …
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Real Time RT-PCR for Direct Detection of Viable Mycobacterium Avium Subspecies paratuberculosis in Chron's Disease Patients and Association of Map Infection with Downregulation in Interferon-Gamma Receptor (INFG1) Gene in Crohn's Disease Patients
… in infected patients. The advent of real time PCR has proven to be significant in infectious disease diagnostics. In this study, real time reverse transcriptase PCR (RT-PCR) assay based on targeting mRNA of the IS900 gene unique to MAP has been developed. All variables included in RNA …
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The WNT signalling pathway in Ewing sarcoma/primitive neuroectodermal tumour : an immunohistochemical investigation
… A recent study on ES cell lines using RT-PCR analysis and biological response assays suggests that an intact WNT pathway exists in ES and that addition of exogenous WNT ligands enhances cell motility. Based on this we hypothesize that the WNT pathway may play a role in the biology of …
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Tetracycline-Associated Regulation of the Transfer Genes of the Bacteroides Conjugative Transposon CTnDOT
… of the CTnDOT transfer (tra) genes. RT-PCR analysis of the region containing the tra genes showed that these genes are transcriptionally coupled in an operon and that expression of this operon is regulated at the transcriptional level. The transcription initiation site of the operon was …
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Tobacco Phospholipase D β1: Molecular Cloning and Biochemical Characterization
… with PLDβ1 from tomato but lacks the C2 domain. PCR analysis confirmed insertion of the antisense fragment into the plants: three introns distinguished the endogenous gene from the transgene. PLD activity was assayed in leaf homogenates in PLDβ/g conditions. When phosphatidylcholine was utilized …
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Studies on the Molecular Biology of Naegleru Fowleri and Identification of N. Fowleri in the Environment
… was conducted to determine whether a nested PCR assay could be used for detection of N. fowleri in freshwater habitats. PCR analysis was used to test samples from Virginia, Connecticut, Arizona, and Oklahoma for the presence of N. fowleri in lakes, ponds, soil, and domestic water supplies. …
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Trophinin: Expression and Regulation in the Porcine Uterus and Embryo During the Time of Embryo Implantation
Reverse Transcriptase (RT) PCR analysis showed that trophinin mRNA was only present in both bred and non-bred porcine uterus. Over 80% of the porcine trophinin gene is homologous to human trophinin. Trophinin was expressed systemically through out the uterus and the presence of an embryo had no …
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Specific diagnosis for effective treatment of neuroblastomas and pulmonary neuroendocrine tumors through transcriptional profiling-based interpretation of cellular development
… polymerase chain reaction (RT-PCR) analysis, the present study compared the expression of about 50 regulatory genes that are expressed at early stages of neurogenesis in five human NBs and NETs, also upon GDNF/TGF-β1 and retinoic acid (RA) treatment. According to our results, …
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Towards plastid transformation in rapeseed (Brassica napus L.) and sugarbeet (Beta vulgaris L.)
… transformants as confirmed by molecular methods (PCR analysis and Southern blot hybridisation). The biolistic method was applied for plastid transformation experiments in sugarbeet. Species specific vectors containing the aadA cassette were constructed for plastid transformation in rapeseed and …
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Establishing a Rodent Model of Postoperative Pain: Investigating Potential Sex Differences and Changes in Gene Expression
… to those in male CFA-treated rats. qRT-PCR analysis of spinal dorsal horn tissue showed no sex differences or surgery-related differences in genes associated with hyperexcitability, plasticity, or inflammation.
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Modification of Vegetable Soybean Sugar Content via Gene Transformation
… transformation and selection were subjected to PCR analysis for the transgenes. Some lines were PCR positive for the hygromycin resistance and/or $\alpha$-galactosidase genes but Southern and northern analysis gave negative results. These results suggest that the transformed genes were lost …
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Characterization of TvDMC1 and TvSOD6 expression and function in trichomonas vaginalis
… protein in homologous recombination, DMCl. RT-PCR analysis shows low expression ofDMCl in mitotically-growing cultures, and we found that some stresses on the organism increase DMCl expression. Polyclonal antibodies raised against DMCl protein have been used to test whole celllysates of the Tl …
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Improved Late-gestation Cardiac Morphology in Fetuses of Diabetic Mothers After Maternal Immune Stimulation: Potential Role of Dysregulated Apoptosis
… the late gestation fetal heart by morphometric analysis. Diabetic females treated with Freund's compete adjuvant (FCA) or interferon-gamma (IFNγ) were also generated to elucidate potential positive effects of maternal immune stimulation during the diabetic pregnancy by morphometric analysis and …
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Effect of culture conditions, donor source, and injection site on in vitro development of deoxyribonucleic acid microinjected porcine zygotes
… were analyzed by the polymerase chain reaction (PCR) for the presence of the transgene. One-cell embryos (n=458) were recovered from 36 postpubertal gilts in Experiment 1. Injected and control embryos were cultured in modified media NCSU-23 (mNCSU-23), NCSU-37 (mNCSU-37), and CZB at 37°C and …
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Characterization Of Pregnancy Induced Cardiac Hypertrophy In Rats
… to their non-pregnant littermates. Histological analysis confirmed an increase in cardiomyocyte size associated with the increase in heart size. Real-time PCR analysis revealed alterations in expression of some gene markers of hypertrophy during pregnancy and postpartum. Our data demonstrates …
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Co-Infection in HIV positive patients with retinitis: A case series of dual positive intraocular fluid polymerase chain reaction
… 10 cases of dual-positive intraocular fluid PCR results in infectious retinitis where both pathogens may be clinically relevant. Methods A retrospective observational case series including 10 patients with infectious retinitis who demonstrated more than 1 positive result on PCR testing over a …
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GENETIC MANIPULATION OF CYTOKININ LEVELS IN NEW PLANT TYPE RICE
… higher than in the leaves of control plants. PCR analysis showed that the ipt gene was inherited in the TI progeny of the transgenic To plants. The ipt gene was inherited in a 3:1 segregation ratio in the TI progeny of transgenic plant No. Tl193 which indicates that the ipt gene was inherited …
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MOLECULAR ANALYSIS OF FLORAL PIGMENTATION IN THE ORCHID, BROMHEADIA FINLAYSONIANA
… with respective polymerase chain reaction (PCR) generated homologous probes. Southern blot analysis revealed that PAL, CHS and F3H are encoded by small multigene families while DFR is represented by a single gene in the genome of Bromheadia. Northern blot and reverse transcriptase-PCR …
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