In Vitro and in Vivo Studies of the Recognition of Double-Strand DNA by EcoRI and RsrI Endonucleases

EcoRI and RsrI endonucleases present rare opportunities to study the mechanisms by which site-specific DNA-binding proteins identify their substrate sequences among an overwhelming number of other sequences to which they also show significant affinity. These proteins have advantages as subjects for the study of protein/DNA recognition in that they are enzymes, permitting the examination of both binding and catalysis. The fact that both enzymes recognize the same sequence and catalyze the same reaction, although they share only 50% amino acid identity and differ physically and chemically, suggests that comparing their recognition properties will be informative with respect to structure/function relationships and to protein evolution. Finally, the effort already expended in characterizing the enzymes leaves them among the best understood of enzymes recognizing specific DNA sequences.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Biochemistry

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2014

Author dc:creator

• Fisher, Eric William

Contributors dc:contributor

• Gumport, Richard I.

• Biology, Genetics
• Biology, Microbiology
• Chemistry, Biochemistry

Identifier

(UMI)AAI9503183

OAI record oai:identifier

https://www.ideals.illinois.edu/oai-pmh?verb=GetRecord&metadataPrefix=etdms&identifier=oai%3Awww.ideals.illinois.edu%3A2142%2F72365