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Showing 1 to 20 of 23 for “"EcoRI"”.
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Studies of sequence recognition by the EcoRI restriction/modification system of Escherichia coli
Sequence recognition by the EcoRI restriction/modification system of Escherichia coli is currently the subject of intense study as a model of the general phenomenon of target location by nucleotide sequence-specific enzymes and binding proteins. Models have proposed a variety of means for site …
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On the Repair of DNA Breaks and the Specificity of the EcoRI Restriction Enzyme
… in E. coli, and substrate recognition by the EcoRI endonuclease. Many species of bacteria make restriction-modification systems to destroy foreign DNA that enters the cell. These systems usually consist of an endonuclease that cleaves a specific DNA sequence and a methylase which modifies the …
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Sequence-specific recognition of DNA by RSRI endonuclease of Rhodobacter sphaeroides, an isoschizomer of EcoRI
… DNA using a mechanism similar to that of EcoRI, I have purified milligram amounts of the enzyme to near homogeneity from its natural source, Rhodobacter sphaeroides strain 630. The enzyme comigrates with EcoRI during SDS-PAGE, with a reduced and denatured molecular weight of 30,000 $\pm$ …
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In Vitro and in Vivo Studies of the Recognition of Double-Strand DNA by EcoRI and RsrI Endonucleases
Made available in DSpace on 2014-12-17T21:58:46Z (GMT). No. of bitstreams: 1 9503183.pdf: 7252488 bytes, checksum: 309c1eef47e382d24902e0bdd38c89ed (MD5) Previous issue date: 1994
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Pathogenicity Genes of Pseudomonas Syringae Pv. Tabaci Br2
… indicated that Tn5 had inserted into different EcoRI and SalI restriction fragments in each of the eight nonpathogenic mutants. Hybridization to additional EcoRI and SalI bands in two mutants indicated a second insertion of IS50 or Tn5. The EcoRI fragment containing Tn5 from each of the eight …
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Molecular analysis of the salmon pathogen Piscirickettsia salmonis
… from the bacteria, partially digested with EcoRI and ligated into ExCell. The library appeared to contain approximately 1.79 x 105 recombinants/g DNA, and the recombinant library was screened with rabbit antiserum to purified P. salmonis and antisera raised in fish. No reactive clones were …
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Low level Tc1 transposition and Tc1-induced mutations in dpy-5
… a novel 2.7 kilobase pair Tcl-hybridizing EcoRI fragment was isolated and used as a tag in cloning the dpy-5 gene. Examination of Tcl-induced mutations of dpy-5 and wild type revertants revealed that the mutation was the result of Tcl transposition into a1.1 kilobase pair genomic EcoRI …
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DNA systematic studies in the genus Bromus L. (Poaceae)
… length heterogeneity within and among species. EcoRI digests were not useful for phylogenetic analysis since the enzyme generally cleaves only once per repeat unit. EcoRI and Kpni endonuclease fragment patterns were used to identify the number of repeat unit length variants per species. The …
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Demonstration of new subtypes of adenovirus 7 in South Africa, and probing oesophageal carcinoma cell lines for evidence of adenovirus or of other oncogenic viruses
… Ad7 c according to restriction profiles with EcoRI; 2 new EcoRI sites were demonstrated in Ad7c1 and 1 in Ad7c2. The restriction sites were mapped on the viral genomes (at 3.68kb and 5.32kb from the left terminus) by double enzyme digestions, cloning of viral DNA, and nucleic acid …
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Strukturelle und funktionale Analyse des DAZL1-Locus in Mammalia
… Die Anzahl der Schnittstellen für BamHI, EcoRI, EcoRV und HindIII hat im Verlauf der Primaten-Evolution zugenommen. Die Homologie der humanen DAZL1-Sonden zu Maus und Rind war für eine Analyse über genomische Southern-Bots zu gering. Es wurden genomische DAZL1-Klone von Mensch, Hylobates …
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Gene cloning studies in two nocardioform bacteria
… site of pLR591 inactivated the lethal EcoRI gene derived from pEcoR251, thereby selecting for recombinant plasmids containing inserted DNA. The thiostrepton resistance gene derived from pIJ702 was shown to be functional in Streptomyces lividans enabling selection of recombinant pLR591 …
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Detection and characterization of antibiotic resistance plasmids in Cheney biosolids
… were digested with a restriction enzyme, EcoRI to verify the presence of multiple plasmids in the samples. The resistant bacteria (13.2%) that showed the presence of plasmid were tested whether they were conjugative or mobilizable type. Unfortunately, none of the isolates were onjugative …
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Cloning and Characterization of a Gene Involved in Lipooligosaccharide Biosynthesis in Haemophilus somnus
… was confirmed using a (CAAT)7 probe. A 3.9 kb EcoRI fragment that reacted with the probe was cloned and sequenced. Sequence analysis confirmed the presence of 31 CAAT repeats downstream of two potential start codons, and indicated that small or large proteins would be encoded depending on the …
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Dynamics and structures of linear and supercoiled DNAs
… of supercoiled and linear DNAs with EcoRI endonuclease, and dye molecules have been studied with DLS experiments. Results of these experiments have been useful in understanding the mechanisms of these reactions.
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Isolierung und Charakterisierung von Iridoviren
… of purified viral DNA with the endonucleases EcoRI, BamHI, and HindIII showed marked differences from the profiles of iridoviruses of lower vertebrates (genus Ranavirus), e.g., Rana esculenta Iridovirus and Frog virus 3. Restriction enzyme digests with the endonucleases MspI and HpaII …
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A molecular biological study on Campylobacter pylori
… but only 3 of the enzymes used digested the DNA. EcoRI and HindIII partially digested the DNA, while Sau3A digested the plasmids completely, generating 2 fragments of 2,2kb and 2,4kb, and a number of smaller fragments. The DNA was shown to be methylated and the fragments generated by Sau3A …
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Diversity and Evolution of Short Interspersed Nuclear Elements (SINEs) in Angiosperm and Gymnosperm Species and their Application as molecular Markers for Genotyping
… from genomic regions between SINEs and EcoRI cleavage sites were visualized with the sensitive capillary gel electrophoresis. The ISRAP assays, based on EcoRI adapter primers combined with two different SINE-derived primers, resulted in a sufficient number of polymorphic peaks to …
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Genetic and Biochemical Analysis of the Bryan Strain of Rous Sarcoma Virus
… determine the defects in this strain. A 5kb EcoRI fragment which contained the entire pol and src genes was molecularly cloned from integrated proviruses of BH-RSV alpha and its pol<sup>+</sup>parent BH-RSV beta. DNA sequencing of the pol-src junction of BH-RSV revealed that the env sequence …
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Biochemical characterization of the nucleic acids of some human and animal viruses
… all four gave the same patterns with EcoRI, BamHI and HindIII. Two isolates that were also digested with PstI gave an identical pattern. These patterns are similar to, but distinct from, other strains of the human polyomavirus BK that have been described. Our isolates had a …
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