Effect of Structure and Composition of Reconstituted High Density Lipoproteins on Phosphatidylcholine Transfer by Human Plasma Phospholipid Transfer Protein

Discoidal-reconstructed high density lipoproteins (rHDL) were used to study the effect of HDL substrates on phosphatidylcholine (PC) transfer by human plasma phospholipid transfer protein (PLTP) by changing the composition and size of rHDL. Sonicated egg-PC:cholesterol vesicles containing radiolabelled dipalmitoyl-PC ($\sp $C-DPPC) were used as donors and varied rHDL, consisting of apolipoprotein, phospholipid and cholesterol, were used as acceptors. PLTP, purified about 3,400-fold, was used in the experiments. Activity was measured by the percent $\sp $C-DPPC transferred from vesicles into rHDL.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Biochemistry

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2014

Author dc:creator

• Williams, Malcolm Irvin

Contributors dc:contributor

• Jonas, Ana

• Chemistry, Biochemistry
• Health Sciences, Nutrition

Identifier

(UMI)AAI8908891

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/72349