Uncovering the Biosynthesis of Natural Products Using Fourier-Transform Mass Spectrometry

Within some of the simplest bacteria there exists the prospect of novel natural products with an exciting array of bioactivities. These natural products are assembled by a vast array of enzymes which can transform the simplest building blocks into large, complex molecules. Fourier-Transform mass spectrometry (FTMS) is a powerful technique for the accurate determination of mass in complex mixtures. A large portion of this research is focused on the multimodular assembly-line enzymes employed in the biosynthesis of polyketides (PK) and nonribosomal peptides (NRP). In this work, FTMS is used both in the discovery of novel natural products as well as in the characterization of novel enzymology.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Chemistry

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2014

Author dc:creator

• Thomas, Paul Martin

Contributors dc:contributor

• Kelleher, Neil L.

• Chemistry, Analytical
• Chemistry, Biochemistry

Identifier

(UMI)AAI3363099

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/72253