Characterization and Regulation of Insulin-Like Growth Factor-Imrna Transcripts in Myeloid Cells

Using reverse transcription and PCR amplification, cDNA fragments corresponding to IGF-I Ea and Eb mRNA transcripts were detected in all murine immune cell types. The identity of these sequences was confirmed by cloning and sequencing. Macrophages had 20 to 40 fold higher levels of IGF-I mRNA than B or T cells and macrophage transcripts initiated exclusively within exon 1, characteristic of extra-hepatic tissues. Surprisingly, macrophage IGF-I mRNA transcripts contained a proportion of IGF-I Eb transcripts which was equal to, or greater than, that produced by the liver. A large molecular weight IGF-I precursor, was detected in macrophage lysates by Western blotting.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Animal Sciences

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2014

Author dc:creator

• Arkins, John Francis

Contributors dc:contributor

• Kelley, Keith W.

• Biology, Molecular
• Biology, Cell
• Health Sciences, Immunology

Identifier

(UMI)AAI9305456

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/72213