Isolation and Characterization of Blood Lipoproteins From Dairy Cows Fed Control or High Grain Diets (Lactation, Feeding, Lipids)

Lipoproteins from the plasma of three non-lactating Holstein cows were isolated using either preparative ultracentrifugation or gel filtration chromatography. Lipoprotein classes obtained by ultracentrifugation were very low density plus chylomicrons, low density, high density(,1), and high density. These lipoprotein classes were applied individually to an agarose gel column in order to determine at what volume they eluted in comparison to lipoproteins that were separated after applying total bovine lipoproteins to the column.Three major peaks corresponding to very low density lipoproteins plus chylomicrons, low density, and high density lipoproteins resulted after gel filtration of total lipoproteins. Very low density lipoproteins plus chylomicrons, obtained by ultracentrifugation, eluted as a single peak as did low density and high density lipoproteins. However, high density lipoproteins 1 eluted as two peaks. The first peak eluted at the same volume as low density lipoproteins while the second peak eluted at a volume similar to that of the ascending slope of the high density lipoprotein peak. Results from disc polyacrylamide gel electrophoresis, immunoelectrophoresis, and double immunodiffusion of lipoprotein fractions, and SDS polyacrylamide gel electrophoresis of their apoproteins, indicated that high density lipoproteins 1 are a mixture of low and high density lipoproteins rather than a unique class of lipoproteins.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Dairy Science

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2014

Author dc:creator

• Grummer, Ric Robert

• Agriculture, Animal Culture and Nutrition

Identifier

(UMI)AAI8422071

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/71722