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Showing 1 to 20 of 139 for “"Ultracentrifugation"”.
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Probing metal nanoparticles and assemblies with analytical ultracentrifugation
Analytical Ultracentrifugation (AUC) is a powerful tool to obtain statistically relevant size and shape measurements for macromolecular systems. Metal nanoparticles coated by a ligand shell of thiolated molecules provide diverse functionality, from targeted cellular delivery to the formation of …
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Identifying novel proteins in translation complex by using analytical ultracentrifugation with fluorescent detection system
… the translation complex, we applied analytical ultracentrifugation with fluorescent detection system (AU-FDS) to detect this complex. Following a one-step affinity purification with Flag-PAB1 using strains carrying translation factors and specific mRNA tagged with GFP, we identified the 78S …
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The Purification and Characterization of The Cytochrome D Containing Terminal Oxidase of Escherichia Coli (Bioenergetics, Membranes, Ultracentrifugation)
The cytochrome d-containing terminal oxidase of Escherichia coli has been purified to at least 90% of homogeneity, judging from the Coomassie blue staining of SDS-polyacrylamide gels. These gels showed that the cytochrome contained two types of subunits with molecular weights of 57,000 Daltons and …
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Rate zonal density gradient ultracentrifugation analysis of repair of radiation damage to the folded chromosome of Escherichia coli
The structure of the membrane-free nucleoid of Escherichia coli and of unfolded chromosomal DNA was investigated by sedimentation on neutral sucrose gradients after irradiation with 60Co gamma-rays and ultraviolet light (2S4nm). Irradiation both in vivo and in vitro was used as a molecular probe of …
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Isolation and Characterization of Blood Lipoproteins From Dairy Cows Fed Control or High Grain Diets (Lactation, Feeding, Lipids)
… cows were isolated using either preparative ultracentrifugation or gel filtration chromatography. Lipoprotein classes obtained by ultracentrifugation were very low density plus chylomicrons, low density, high density(,1), and high density. These lipoprotein classes were applied individually …
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Physical and biochemical studies of histone H1 and its globular domain
… scattering, equilibrium density and velocity ultracentrifugation, and chemical crosslinking.
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Genetic polymorphism of the human apolipoproteins A and E
… Polymorphic forms of apo E were detected after ultracentrifugation of plasma, delipidation of VLDL and isoelectric focusing of the remaining protein. The six common phenotypes, homozygotes E 2/2, E 3/3, E 4/4 and the heterozygotes E 2/3, E 2/4, E 3/4, of apo E were demonstrated. A simplified one …
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Compaction of chromatin in Saccharomyces cerevisiae
… fractionated and isolated by sucrose gradient ultracentrifugation from stationary and exponential phase cultures was compared using genome-wide technologies. This study showed that condensed chromatin of stationary phase culture contained an enriched density of genes on all the chromosomes, …
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Cell-Cycle Specific Biosynthesis of the Photosynthetic Membrane of Rhodopseudomonas Sphaeroides: Structural Implications
… by the use of polyethylene glycol as fusogen. Ultracentrifugation, electron microscopy (EM), intrinsic density and isotope labeling were used to demonstrate chromatophore fusion. Except increased ion permeability, the orientation and integrity of fused chromatophores were demonstrated to be …
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Nonideality in high concentration solutions
… framework was sufficient to explain analytical ultracentrifugation data gathered in complex biological solutions. Additional hypotheses tested were as follows: The Fluorescence Detection System for the Analytical Ultracentrifuge will enable the tracking of a single component is a complex …
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Investigations into the digestive system proteases of Argiope aurantia (Araneae: Araneidae), hemolytic agents of spider venoms, and the role of both in necrotic arachnidism
… chromatography, size exclusion chromatography, ultracentrifugation, and electrophoresis, an attempt was made to isolate several digestive fluid proteases, and seine protease inhibitors in milligram quantities from the digestive fluid of Argiope aurantia . The proteases, however, eluted …
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ROLE OF APOLIPOPROTEIN A-V IN TRIGLYCERIDE METABOLISM
… and its flotation during sucrose gradient ultracentrifugation. The possible intracellular role of apoA-V in the regulation of apoB and TG secretion was examined by generating stably transfected doxycycline-inducible McA-RH7777 cells. Upon induction of apoA-V expression, a 32% decrease in …
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Development of a plant-made immunoassay for the detection of Porcine circovirus infections in South African swine herds
… chromatography (IEC) and sucrose gradient ultracentrifugation were explored to obtain pure VLPs free of bacterial contamination. The VLPs were successfully expressed in N. benthamiana with both pEAQ-HT and pCBP2, and VLPs were subsequently purified on discontinuous sucrose gradients by …
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ΚΙΝΑΣΗ ΤΗΣ ΦΩΣΦΟΡΥΛΑΣΗΣ ΑΠΟ ΛΕΙΟ ΜΥΙΚΟ ΙΣΤΟ ΣΤΟΜΑΧΙΟΥ ΚΟΤΑΣ: ΑΠΟΜΟΝΩΣΗ ΚΑΙ ΜΕΛΕΤΗ ΤΩΝ ΚΑΤΑΛΥΤΙΚΩΝ ΚΑΙ ΔΟΜΙΚΩΝ ΙΔΙΟΤΗΤΩΝ ΤΗΣ
… SEPHAROSE 4B AFFINITY CHROMATOGRAPHY COLUMN AND ULTRACENTRIFUGATION INGLYCEROL GRADIENT DENSITY CHICKEN GIZZARD PHOSPHORYLASE KINASE PRESENTS DIFFERENT STRUCTURAL AND CATALYTIC PROPERTIES IN DELATION TO THOSE FOUND FOR THE RABBIT SKELETAL MUSCLE ISOENZYME. ALTHOUGH BOTH KINASES HAVE THE SAME HIGH …
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Development of in vitro Chylomicron Assay Using Caco-2 Cells
… that may affect the secretion of CMs through the ultracentrifugation technique. The dose-dependent effects of oleic acid, mono-olein, egg lecithin, collagen matrix, and the effect of cell differentiation on CM secretion were then tested. We found that oleic acid, lecithin, and cell differentiation …
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Effects of The Acta2 R258C Mutation On Vascular Smooth Muscle Cell Phenotype and Properties
… Factor A (MRTF-A) localization. However, ultracentrifugation assays showed large unpolymerized actin fractions, suggesting that the filaments are brittle. In contrast, <em>Acta2<sup>+/- R258C TG</sup></em> SMCs are less well-differentiated, with pools of unpolymerized actin, more …
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The use of non-ionic surfactant vesicles to prevent radiocontrast media-associated damage
… measure surface charge. NIVs were purified using ultracentrifugation prior to the quantification of IOX encapsulation efficiency and release using high performance liquid chromatography. Toxicity of IOX, empty-NIV and IOX-NIV was tested on renal and vascular cells using assays including the …
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Apolipoprotein E Activation and Phosphatidylcholine Substrate Requirements of Lecithin Cholesterol Acyl Transferase
… when the unbound apolipoprotein was removed by ultracentrifugation and reisolated complexes were assayed.
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