Molecular Genetic Analysis of Rhodobacter Sphaeroides Light-Harvesting Complexes

The genes for the Rhodobacter sphaeroides light-harvesting, B875-β and B875-α polypeptides (pufB and pufA) are closely linked to the genes for the reaction center-L and reaction center-M polypeptides (pufL and pufM) on what has been termed the puf operon (gene order pufB,A,L,M). The DNA sequence of pufB and pufA from wild-type R. sphaeroides 2.4.1 was determined. The relative levels of the B875-β and B875-α and the reaction center-L and reaction center-M polypeptides synthesized in a homologous cell-free transcription-translation system were compared with those found in vivo. Analysis of the gene products produced in vitro using plasmids containing deletions upstream of the pufB structural gene identified a region of DNA required for expression of the B875-β and B875-α polypeptides. These results support the hypothesis that the mapped 5$\sp\prime$ termini of the large and small puf operon transcripts represent transcription initiation sites.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Microbiology

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2014

Author dc:creator

• Kiley, Patricia Jeannette

Contributors dc:contributor

• Kaplan, Samuel

• Biology, Microbiology

Identifier

(UMI)AAI8803085

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/71187