Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 83 for “"Transcription initiation"”.
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Regulation of transcription initiation at guanine + cytosine-rich promoters.
Regulation of transcription initiation at guanine + cytosine-rich promoters.
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Expression and initial characterisation of the Plasmodium falciparum general transcription factors TFIIB and TLP
… largely regulated via RNA Polymerase II (RNAPII) transcription initiation, but in P. falciparum the underlying mechanisms have not been determined. This primarily because very little is known about both the key protein factors and DNA elements which guide the assembly of RNAPII components into the …
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Transcription regulation in Plasmodium falciparum : functional characterisation of general transcription factor IIB
… In eukaryotes, regulation of RNA Polymerase II transcription initiation is one of the main mechanism of gene expression control. Past research has revealed the presence of crucial elements of RNA Polymerase II (RNAPII) transcription machinery in P. falciparum, however, the precise transcription …
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Sequence and Functional Structures of the Extrachromosomal rDNA Minichromosome of Physrum Polycephalum
… region of the rDNA previously identified as the transcription initiation site was cloned in Charon 28 bacteriophage. The initial large clone was subcloned in M13mp9, and a small clone containing the initiation site was isolated. The transcription initiation site was mapped within this clone using …
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Transcriptional Control during Quorum Sensing by LuxR and LuxR Homologues
… lux box centered at à 42.5 bp upstream of the transcription initiation site and activates transcription from the lux operon promoter, resulting in the emission of light at high cell densities. LuxR consists of 250 amino acids arranged into an N-terminal (regulatory) domain and a C-terminal …
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Regulation of transcription in Plasmodium falciparum, the causative agent of severe malaria: initial characterisation of PfTBP and PfTFIIA
… and function of the RNA polymerase II (RNAP-II) transcription initiation complex composed of RNAP-II and the general transcription factors (GTFs). Bioinformatics analyses show that the RNAP-II GTFs in P. falciparum have greatly diverged from those studied in other eukaryotes, suggesting the …
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Regulation of Gene Expression in Trypanosoma brucei
… II (RNA pol II) are arranged in polycistronic transcription units (PTUs). mRNAs are separated post-transcriptionally by coupled splicing and polyadenylation reactions. During the splicing reaction a 39 nt 'sliced-leader' is added to every mRNA, a process termed trans splicing. The arrangement …
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Analysis of tandemly and divergently oriented spinach chloroplast promoters
… Part of this heterogeneity in RNA is due to posttranscriptional RNA processing events; however, some chloroplast genes such as the $\beta$ subunit of the ATP synthase (atpB) may contain multiple promoters. Ba131 deletion analyses have determined that 3 out of 4 of the previously observed spinach …
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A Molecular Characterization of Human General Transcription Factor IID
<p>The general transcription initiation factor IID plays a central role in transcriptional control as a direct target for a diverse array of gene-specific regulatory factors and as the only template-bound class II initiation factor. The work described in this thesis concerns itself with the …
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Characterization of phenobarbital inducible rabbit cytochrome P450IIC4 and related genes
… flanking region of P450IIC4, the major transcription initiation site was assigned by mung bean nuclease protection mapping to an ""A"", 22 base pairs upstream from the translation initiation codon. A ""TATA"" sequence, TATAAA, was found 25 base pairs further upstream from the …
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Cryo-EM Studies of Bacterial RNA Polymerase
… a single RNA polymerase (RNAP) performs all transcription. The overall structure of bacterial RNAP resembles a crab claw with pincers comprising the β' and β subunits and a large cleft where the active site sits. Structural information about this essential enzyme has mainly been provided by …
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Regulation of Pituitary Genes by the Transcription Factor, Pit-1, in the Domestic Turkey (A Turkey is NOT a Feathered Rat)
The transcription factor, Pit-1, is involved in the transcriptional regulation of the mammalian prolactin (Prl), growth hormone (GH) and thyroid stimulating hormone b-subunit genes (TSHb) as well as its own gene. The role of Pit-1 in avian species is unknown. Three turkey (t) Pit-1 isoforms have …
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Tetracycline-Associated Regulation of the Transfer Genes of the Bacteroides Conjugative Transposon CTnDOT
… the tra genes showed that these genes are transcriptionally coupled in an operon and that expression of this operon is regulated at the transcriptional level. The transcription initiation site of the operon was located 290 bp upstream of the start codon of the first tra gene, traA.
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Enzymatic Disassembly of Promoter Bound 7SK snRNP Drives Transcription Elongation of HIV and Cellular Genes
… genes (PRG’s) is regulated at the step of transcription elongation. Shortly after transcription initiation, RNA Polymerase II (Pol II) pauses and it only enters into productive elongation after inducible transcription factors (TF’s) recruit the P-TEFb kinase to phosphorylate Pol II in …
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Structural and Functional Studies of Mycobacterial General Transcription Factors RbpA and CarD
… in bacteria is highly regulated at the step of transcription initiation. For decades, the vast majority of biochemical and kinetic studies have used Escherichia coli (Eco) RNA polymerase (RNAP) as a model for studying prokaryotic transcription initiation. However, properties of Eco RNAP are not …
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Genome - Wide Regulatory Principles of Essential Transcription Factors from Mycobacterium Tuberculosis
… stored in DNA into RNA, a process known as transcription. Hence, DNA-dependent RNA polymerases (RNAPs) perform the first step of gene expression, thereby dictating the amounts of gene products that proceed to downstream cellular reactions. The activity of RNAP can be modulated by numerous …
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Regulation of transcription of SerA in Escherichia coli K-12
… Previous studies have determined two different transcription initiation sites for the serA gene, corresponding to the promoters of serA P1 and P2, and suggested that P1 and P2 were regulated by leucine responsive regulatory protein (Lrp) by different mechanisms. However, very little is known …
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Molecular Genetic Analysis of Rhodobacter Sphaeroides Light-Harvesting Complexes
… synthesized in a homologous cell-free transcription-translation system were compared with those found in vivo. Analysis of the gene products produced in vitro using plasmids containing deletions upstream of the pufB structural gene identified a region of DNA required for expression of …
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