Cell-Cycle Specific Biosynthesis of the Photosynthetic Membrane of Rhodopseudomonas Sphaeroides: Structural Implications

Fusion of chromatophores, the photosynthetic membrane vesicle isolated from the intracytoplasmic membranes (ICM) of the photosynthetic bacterium Rhodopseudomonas sphaeroides, was achieved by the use of polyethylene glycol as fusogen. Ultracentrifugation, electron microscopy (EM), intrinsic density and isotope labeling were used to demonstrate chromatophore fusion. Except increased ion permeability, the orientation and integrity of fused chromatophores were demonstrated to be identical to control chromatophores by freeze-fracture EM, proteolytic enzyme digestion, enzymatic radioiodination, and transfer of chromatophore phospholipids mediated by phospholipid exchange protein extracted from R. sphaeroides.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Microbiology

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2014

Author dc:creator

• Yen, Grace Shau-Ling

• Biology, Microbiology

Identifier

(UMI)AAI8410078

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/71165