Motility and Chemotaxis of Erwinia Herbicola and Its Effect on Erwinia Amylovora (Biological Control, Phytobacteriology, Fire Blight)

Optimum conditions required for motility and chemotaxis in Erwinia herbicola (112Y) were determined by the capillary assay. Actively motile cells were selected from the periphery of swarming colonies grown on soft agar (0.25%) modified Emerson's medium plates minus the ingredient nutrient broth. The optimum growth temperature for producing motile cells was 29 C. Cells were still motile at assay temperatures of 37 C. Good motility was observed when E. herbicola cells were suspended in a motility medium consisting of 10('-2)M K(,2)HPO(,4)-KH(,2)PO(,4) buffer, 10('-3)M ethylene-diamine tetraacetic acid (EDTA) and adjusted to pH 7. Motility occurred without an exogenous energy source; however, mannitol at 10('-2)M, slightly enhanced motility after induction in modified Miller and Schroth's medium.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Plant Pathology

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2014

Author dc:creator

• Klopmeyer, Michael Jon

• Agriculture, Plant Pathology

Identifier

(UMI)AAI8521806

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/70602