Motility and Chemotaxis of Erwinia Herbicola and Its Effect on Erwinia Amylovora (Biological Control, Phytobacteriology, Fire Blight)
Optimum conditions required for motility and chemotaxis in Erwinia herbicola (112Y) were determined by the capillary assay. Actively motile cells were selected from the periphery of swarming colonies grown on soft agar (0.25%) modified Emerson's medium plates minus the ingredient nutrient broth. The optimum growth temperature for producing motile cells was 29 C. Cells were still motile at assay temperatures of 37 C. Good motility was observed when E. herbicola cells were suspended in a motility medium consisting of 10('-2)M K(,2)HPO(,4)-KH(,2)PO(,4) buffer, 10('-3)M ethylene-diamine tetraacetic acid (EDTA) and adjusted to pH 7. Motility occurred without an exogenous energy source; however, mannitol at 10('-2)M, slightly enhanced motility after induction in modified Miller and Schroth's medium.
University of Illinois - Urbana-Champaign
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University of Illinois at Urbana-Champaign