Motility and Chemotaxis of Erwinia Herbicola and Its Effect on Erwinia Amylovora (Biological Control, Phytobacteriology, Fire Blight)
type: CreativeWork
description: Optimum conditions required for motility and chemotaxis in Erwinia herbicola (112Y) were determined by the capillary assay. Actively motile cells were selected from the periphery of swarming colonies grown on soft agar (0.25%) modified Emerson's medium plates minus the ingredient nutrient broth. The optimum growth temperature for producing motile cells was 29 C. Cells were still motile at assay temperatures of 37 C. Good motility was observed when E. herbicola cells were suspended in a motility medium consisting of 10('-2)M K(,2)HPO(,4)-KH(,2)PO(,4) buffer, 10('-3)M ethylene-diamine tetraacetic acid (EDTA) and adjusted to pH 7. Motility occurred without an exogenous energy source; however, mannitol at 10('-2)M, slightly enhanced motility after induction in modified Miller and Schroth's medium.
abstract: Optimum conditions required for motility and chemotaxis in Erwinia herbicola (112Y) were determined by the capillary assay. Actively motile cells were selected from the periphery of swarming colonies grown on soft agar (0.25%) modified Emerson's medium plates minus the ingredient nutrient broth. The optimum growth temperature for producing motile cells was 29 C. Cells were still motile at assay temperatures of 37 C. Good motility was observed when E. herbicola cells were suspended in a motility medium consisting of 10('-2)M K(,2)HPO(,4)-KH(,2)PO(,4) buffer, 10('-3)M ethylene-diamine tetraacetic acid (EDTA) and adjusted to pH 7. Motility occurred without an exogenous energy source; however, mannitol at 10('-2)M, slightly enhanced motility after induction in modified Miller and Schroth's medium.
author: Klopmeyer, Michael Jon
keywords: Agriculture, Plant Pathology
date published: 2014-12-15T23:52:21Z
date modified: 2026-07-22T22:26:03Z
provider: University of Illinois - Urbana-Champaign
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: University of Illinois at Urbana-Champaign

PropertyValue
property ID: Handle
value: http://hdl.handle.net/2142/70602