Detection of Bean Golden Mosaic Virus in Host Tissues and Infectivity of Its Genome in Bean Mesophyll Protoplasts

Indirect fluorescent antibody (F-Ab) staining, enzyme-linked immunosorbent assay (ELISA), and DNA-DNA hybridization on nitrocellulose (dot blot) were used to detect BGMV in P. vulgaris (Top Crop). F-Ab staining of sections from leaves, stems, and roots showed that viral antigen was present in a limited number of mesophyll cells, in some sieve elements and phloem parenchyma of trifoliolate leaves, and in sieve elements and phloem parenchyma of the stem. No viral antigen was detected from the roots. Isolated cells from trifoliolate leaves showed BGMV-specific fluorescence in the nucleus and cytoplasm. By ELISA, virus titers in trifoliolate leaves reached a maximum of 73 to 125 (mu)g per gram tissue between 6 to 18 days after inoculation (DAI) of the primary leaves. Maximum titers in stems were 1.0 to 1.8 (mu)g per gram tissue between 16 to 22 DAI, and in inoculated primary leaves 0.10 to 0.15 (mu)g virus per gram between 18 to 26 DAI. Dot blots of sap or nucleic acid extracts from trifoliolate leaves showed signals even well into the chronic infection stage when viral titer by ELISA was low. These results suggest that BGMV genome exists in some stable form other than as an intact virion in chronically diseased plants.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Plant Pathology

Grantor

University of Illinois at Urbana-Champaign

Date dc:date

10000-01-01

Author dc:creator

• Bajet, Narceo Bosque

• Agriculture, Plant Pathology

Identifier

(UMI)AAI8409856

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/70593