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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 57 for “"Protoplasts"”.
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Rna Metabolism of Phage Infected Protoplasts
Made available in DSpace on 2014-12-05T21:45:31Z (GMT). No. of bitstreams: 1 5904489.pdf: 3862437 bytes, checksum: 71fdf36b2a559646088a160e5e6077d9 (MD5) Previous issue date: 1959
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Antioxidant responses of pea (Pisum sativum L.) protoplasts
Freshly isolated protoplasts from pea leaves were used to investigate the responses of antioxidant enzymes to oxidative stress. Two cultivars, Progress (tolerant) and Nugget (sensitive), that have differing resistance with respect to oxidative stress at the whole plant level were used. Sulfite and …
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Effects of pH on the osmotic properties of Bacillus megaterium protoplasts
Thesis (M.S.)--Michigan State University. Dept. of Microbiology and Public Health, 1975
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Cellulase and related enzyme activity in protoplasts and mycelium of filamentous fungi.
… factors affecting the production and release of protoplasts from the closely related A. fumigatus and A. niger and P. ochrochloron and G. emersonii were investigated. Protoplast yields were found to be greatly dependent upon growth temperature, cultural conditions, mycelial age, mycelial …
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Factors influencing transient gene expression in electroporated tall fescue (Festuca arundinacea Schreb.) protoplasts
… transient gene expression were studied in protoplasts, after the reporter β-glucuronidase (GUS) gene was introduced by electroporation. In a time-course study of transient gene expression, GUS activity peaked at 24 h after electroporation. Among the different field strength conditions …
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Detection of Bean Golden Mosaic Virus in Host Tissues and Infectivity of Its Genome in Bean Mesophyll Protoplasts
Indirect fluorescent antibody (F-Ab) staining, enzyme-linked immunosorbent assay (ELISA), and DNA-DNA hybridization on nitrocellulose (dot blot) were used to detect BGMV in P. vulgaris (Top Crop). F-Ab staining of sections from leaves, stems, and roots showed that viral antigen was present in a …
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Mixed infections of maize dwarf mosaic virus and cucumber mosaic virus in maize
… padi and Rhopalosipbum maidis aphids. Protoplasts were isolated from maize seedlings and could be viably maintained for up to 66 hours. The maize protoplasts were infected with CMV and MDMV either singly, or together as a mixed inoculum. Infection curves for each virus were plotted. The …
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Towards plastid transformation in rapeseed (Brassica napus L.) and sugarbeet (Beta vulgaris L.)
… transformation in both species. Tobacco leaf protoplasts (cv. ”petite Havana”, cv. ”Wisconsin 38”) were used to develop a novel technique – the TAL (thin-alginate-layers) technique. The TAL technique in combination with new culture media resulted in very rapid protoplast development and fast …
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A CHARACTERIZATION OF THE BACTERIOPHAGE 41C AND ITS LYTIC CYCLE IN BACILLUS SUBTILIS 168
… cells were treated with lysozyme to form protoplasts. However, phage were never found within the extruded mesosomes. Furthermore, phage replication occurred in protoplasts, although the burst size was decreased. Sucrose-stabilized protoplasts only produced a burst of 1-2% of that in whole …
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Effect of Liposome Encapsulation of Cauliflower Mosaic Virus and Its Dna on Plant Infectivity and Culture of Turnip (Brassica Rapa L.) Protoplasts, Their Uptake and Expression of Exogenous Dna
An infectivity test was developed for assessing the effect of liposome encapsulation of cauliflower mosaic virus (CaMV) and its DNA. When grown at temperatures below 24 C, inoculated half-leaves of turnip (Brassica rapa L. cv. Just Right) produced distinct lesions. Decolorizing and staining leaves …
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Application of molecular markers to characterize potato plants derived from anther culture and protoplast fusion
… due to the effect of the "monoploid sieve" Protoplasts are plant cells, the cell walls of which have been digested by a combination of pectinase and cellulase enzymes. If the protoplasts are cultured in a suitable medium, they can resynthesize the cell walls, divide and regenerate complete …
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Developing and applying transient expression systems to identify and understand gene regulatory elements in plants
… transformation protocol for maize mesophyll protoplasts. Using this protocol, I can transform millions of maize mesophyll protoplasts without losing viability. I use transient transformation of maize protoplasts along with Agrobacterium-mediated transient transformation in tobacco to develop …
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Identification Of Heavy Metal Transporters
… of interest through RNA interference in plant protoplasts. The protocol has been developed for Arabidopsis thaliana; however, since protoplasts can be isolated from different tissues and different plant species, direct transfer of synthetic double stranded RNA into protoplasts can be employed …
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Development of a genetic transformation system in the family Ceratocystidaceae with specific reference to Ceratocystis albifundus
… relies on the successful production of viable protoplasts, and a protocol was created to produce these from C. albifundus conidia. When these protoplasts were used for transformation with PEG/CaCl2-protoplast protocols, no transformants were obtained in spite of the many alterations that were …
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Bioenergetics of Methanococcus voltae: Possible role for a primary sodium pump in ATP formation
… a chemiosmotic mechanism. Results obtained with protoplasts from Methanobacterium thermoautotrophicum also agree with a chemiosmotic mechanism of ATP synthesis. Lancaster proposed a mechanism of ATP formation in M. voltae coupled to a novel scheme of substrate level phosphorylation. The …
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The functional characterisation of the XhABFA transcription factor from the resurrection plant Xerophyta humilis
… transient expression of XhABFA in A. thaliana protoplasts transfected with promoter:firefly luciferase reporter constructs, and a Yeast One-Hybrid analysis. A. thaliana protoplasts expressing XhABFA displayed significantly greater firefly luciferase activity than protoplasts transfected with …
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The Use of Morphogenic Transcription Factors to Enhance Plant Regeneration
… transgenic methods of gene editing is the use of protoplasts (cells whose cell wall has been removed) for transient expression and subsequent regeneration of non-GMO, edited plants. However, efficient regeneration of plants from protoplasts is a bottleneck in the implementation of this technique. …
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Advancing plant transformation and CRISPR/Cas editing: Strategies for recalcitrant maize
… isolation and the effective application of protoplasts for CRISPR/Cas editing. We offered a detailed, sequential protocol for isolating mesophyll protoplasts from in vitro cultured pennycress and soil-grown camelina. The protocol encompasses instructions for both DNA transfection and …
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The Genome of Bean Golden Mosaic Virus: Restriction Map and Use as a Probe to Sensitively Diagnose Geminivirus Infection
… studies of dose/infection relationships in bean protoplasts inoculated with BGMV DNA suggest that the genome is divided between two components encapsidated in separate geminate particles.
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