Metabolism of Heparan Sulfate Proteoglycan in Rat Hepatocyte Cell Line and Its Role for Regulation of Cell Growth

A rat hepatocyte cell line which accumulates free heparan sulfate (HS) chains in the nucleus was labeled with $\sp{35}$SO$\sb4\sp{2-}$ and the rate of appearance of ($\sp{35}$SO$\sb4$) HS in the nucleus was measured. ($\sp{35}$SO$\sb4$) HS began to accumulate in the nucleus 2 h after the addition of $\sp{35}$SO$\sb4\sp{2-}$ and reached a steady state level after 20 h. HS was lost from the nuclei of pre-labeled cells with a t$\sb{1/2}$ of 8 h. At both 37$\sp\circ$C and 16$\sp\circ$C exogenous ($\sp{35}$SO$\sb4$) heparan sulfate proteoglycan (HSPG) was taken up by the cells and converted to free chains and about 10 percent of the internalized HS was transported into nucleus.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Biochemistry

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2014

Author dc:creator

• Ishihara, Masayuki

• Chemistry, Biochemistry

Identifier

(UMI)AAI8823153

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/70569