Isolation and Characterization of the Chloroperoxidase Gene From Caldariomyces Fumago

The chloroperoxidase gene (cpo gene) from the filamentous fungus, Caldariomyces fumago, was isolated within a 16.3 kilobase DNA fragment in the vector LambdaEMBL3. The DNA sequence of the gene and its proximal flanking regions was determined and analyzed by using the programs available on the BIONET National Computer Resource for Molecular Biology. Such analysis provided the primary sequence of the protein and identified regions of DNA sequence which have structures related by symmetry operations. By analogy with the structure of sequences known to function in the control of gene expression, these regions may be those responsible for the observed expression properties of the cpo gene. The actual function of DNA sequences in the flanking regions remains to be determined. Comparison of the genomic and cDNA versions of the cpo gene sequence shows that the gene lacks introns and does not have the typical AATAAA polyadenylation signal. The start site of transcription was mapped by primer extension analysis and found conform to the general pattern of eukaryotic transcription initiation sites. A search of a portion of the BIONET databases for sequences similar to that of the cpo gene did not find any significant matches.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Biochemistry

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2014

Author dc:creator

• Nuell, Mark Jay

• Chemistry, Biochemistry

Identifier

(UMI)AAI8701579

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/70559