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Showing 1 to 20 of 22 for “"Chloroperoxidase"”.
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Expression of Recombinant Chloroperoxidase
Chloroperoxidase from the filamentous fungus Caldariomyces fumago is a heme protein with a unique structure and broad catalytic activities. In this thesis research, we report on the development of protein expression systems for chloroperoxidase. Both prokaryotic and eukaryotic expression systems …
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Enantioselective Epoxidation of Alkenes by Chloroperoxidase and the Development of a Chloroperoxidase Expression System
Chloroperoxidase from Caldariomyces fumago has the characteristics of a promising industrial catalyst. Chloroperoxidase is inexpensive to produce and relatively stable and able to catalyze a large number of potentially useful reactions. However, the majority of the reactions studied occur with a …
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Active-Site Studies on Chloroperoxidase
Made available in DSpace on 2014-12-10T23:24:34Z (GMT). No. of bitstreams: 1 7414568.pdf: 4848976 bytes, checksum: 2faa69730463db88f4a2a8aba3309fc7 (MD5) Previous issue date: 1974
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Crystallization and Properties of Chloroperoxidase
Made available in DSpace on 2014-12-09T17:35:15Z (GMT). No. of bitstreams: 1 6507140.pdf: 4104071 bytes, checksum: 1088fb0013b7755db17796b2898838a7 (MD5) Previous issue date: 1965
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Studies of the Expression of Chloroperoxidase: Isolation and Sequence of a Chloroperoxidase Cdna Clone (complementary-Dna)
… fungus Caldariomyces fumago secretes the enzyme chloroperoxidase at levels exceeding 400 milligrams per liter of culture medium when fructose is provided as the carbon source. Harnessing the cellular machinery which produces chloroperoxidase could provide a powerful method for the production and …
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Oxidation of Alkenes by Caldariomyces Fumago Chloroperoxidase
To elucidate factors involved in the control of CPO heme N-alkylation by terminal alkenes, the epoxidation of a series of monosubstituted and 1,1-disubstituted terminal alkenes by CPO was investigated. Terminal alkenes containing a 2-methyl substituent did not inactivate CPO even when the …
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The Chemical Nature of Chloroperoxidase Compound I
Made available in DSpace on 2014-12-14T04:27:38Z (GMT). No. of bitstreams: 1 7726661.pdf: 3935962 bytes, checksum: b573bf3c0101b7ce7b4bebae1d44b7c6 (MD5) Previous issue date: 1977
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Studies on the Mechanism and Active Site of Chloroperoxidase
Made available in DSpace on 2014-12-09T17:35:44Z (GMT). No. of bitstreams: 1 6711828.pdf: 4745016 bytes, checksum: 80cbd88de86534b6ef6a7665632c4ae3 (MD5) Previous issue date: 1967
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Isolation and Characterization of the Chloroperoxidase Gene From Caldariomyces Fumago
The chloroperoxidase gene (cpo gene) from the filamentous fungus, Caldariomyces fumago, was isolated within a 16.3 kilobase DNA fragment in the vector LambdaEMBL3. The DNA sequence of the gene and its proximal flanking regions was determined and analyzed by using the programs available on the …
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Characterization of the Active Site of Chloroperoxidase Using Physical Techniques (Hemeproteins)
Chloroperoxidase (CPO) and Cytochrome P-450, two very different hemeproteins, have been shown to have similar active sites by several techniques. Recent work has demonstrated thiolate ligation from a cysteine residue to the iron in P-450. A major portion of my research has been devoted to obtaining …
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Cloning, Sequencing and Expression of Chloroperoxidase Complementary-Dna in Escherichia Coli
Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1988.
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Studies on the Mechanism of the Halogenation Reactions Catalyzed by Chloroperoxidase
Made available in DSpace on 2014-12-09T17:36:18Z (GMT). No. of bitstreams: 1 6910864.pdf: 6006461 bytes, checksum: 29370b4f6e2f2dbbaaf3b6c21b14556c (MD5) Previous issue date: 1968
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Delineation of the active site structure of chloroperoxidase from C. fumago
Chloroperoxidase, a heme-containing glycoprotein from Caldariomyces fumago, is secreted in prodigious amounts in the presence of fructose. Production of 90% pure, mycelium-free enzyme for 200 days from a single inoculum was facilitated by continuous and semi-continuous bioreactors designed and …
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The Free Radical Nature of Compound I From Horseradish Peroxidase and Chloroperoxidase
… were prepared from Horseradish Peroxidase (HRP), Chloroperoxidase (CPO), mesohemin substituted Horseradish Peroxidase (M-HRP) and deuterohemin substituted Horseradish Peroxidase. The Compound Is were analyzed by visible absorption, electron paramagnetic resonance (EPR), Mossbauer and electron …
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Studies on the Mechanism of the Chlorination Reactions Catalyzed by Chloroperoxidase and by Horseradish Peroxidase With Chlorite
Chloroperoxidase catalyzes the dismutation of chlorite-forming chloride, chlorine dioxide, chlorate, and oxygen as products. Chloroperoxidase also catalyzes the decomposition of chlorine dioxide. Chloride, chlorate, and oxygen are the products of the decomposition of chlorine dioxide. The optimum …
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Development of a Transformation System for Caldariomyces Fumago and Expression of Heterologous Proteins
… is a filamentous fungus that secretes the enzyme chloroperoxidase in amounts greater than 1 g/l at a purity level of 90-95% on a simple fructose sugar/inorganic salts medium. Conditions were optimized for the generation of spheroplasts from C. fumago mycelium using the enzyme mixture Novozym 234. …
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Control of ligand binding to heme proteins: The role of the distal histidine
… on the effect of pH on the CO recombination to chloroperoxidase show a reversed effect. The rates increase with increasing pH, unlike myoglobin and beta hemoglobin. However, the pK and energy difference between the protonated and unprotonated states suggest a distal histidine is also being …
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Purification and characterization of methyl chloride transferase: A novel halogenating enzyme
… common to haloperoxidase enzymes such as chloroperoxidase. No production of monohalomethanes can be detected by the haloperoxidase mechanism. The white rot fungus, Phellinus pomaceus has been known to produce methyl chloride in vivo. After determining appropriate growth conditions for …
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Chloroplast Biotechnology in Higher Plants: Expressing Antimicrobial Genes in the Plastid Genome
… by host cytoplasmic proteases. A bacterial chloroperoxidase gene (cpo-p) was transformed into the tobacco chloroplast genome to test its efficacy against plant pathogens and the mycotoxin producing saprophyte Aspergillus flavus.
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Relaxation dynamics in heme proteins
… and substrate-free cytochrome P450cam-CO, chloroperoxidase-CO, horseradish peroxidase-CO) between 150 K and 250 K using FTIR spectroscopy to monitor the CO bound to the heme iron. Two types of prelaxation experiments are performed: p-~elease {200 ___. ~ 40 MPa) and p-jump (~ 40 ___. 200 …
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