Interaction of Human Apolipoprotein a-I With Dipalmitoyl Phosphatidylcholine

Dipalmitoylphosphatidylcholine (DPPC) bilayer lipid will react with human apolipoprotein A-I (apo A-I) to form lipid-protein micellar complexes. The reactivity of the lipid bilayer is dependent upon the physical state of the vesicle. Large vesicles with essentially planar bilayers do not form complexes except at temperatures near the gel to liquid-crystalline phase transition of the vesicles where they demonstrate a slow rate of complex formation. Small unilamellar vesicles which contain bilayers with a high degree of surface curvature are rapidly incorporated into micellar complexes at temperatures near and above the transition temperature. The highly reactive nature of the small vesicles is explained by the marked curvature of the lipid bilayer which affects the packing of the DPPC molecules in the bilayer and facilitate its penetration by apo A-I.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Biochemistry

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2014

Author dc:creator

• Wetterau, John Rowley, Ii

• Chemistry, Biochemistry

Identifier

(UMI)AAI8303021

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/70515