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University of Illinois at Urbana-Champaign
The Substrate Specificity of Testicular Hyaluronidase and Its Use in Sequencing Chondroitin Sulfates
Abstract
dc:descriptionTesticular hyaluronidase was used to study the distribution of sulfated disaccharide units along chondroitin sulfate chains. The transglycosylation activity of the hyaluronidase was eliminated by use of excess levels of a second enzyme, (beta)-glucuronidase to remove all nonreducing terminal D-glucuronic acid residues from oligosaccharides present in the reaction mixture. This action prevented the oligosaccharides from participating in the transglycosylation reaction. Conditions were also designed to eliminate sulfatase activities present in the hyaluronidase preparation. the D-glucuronic acid released also provided a convenient assay of the hyaluronidase activity.
Degree
thesis:*- Name thesis:degree_name
- Ph.D.
- Level thesis:degree_level
- Dissertation
- Discipline thesis:degree_discipline
- Biochemistry
- Grantor
- University of Illinois at Urbana-Champaign
- Year dc:date
- 2014
Author and committee
dc:creator, dc:contributor.*- Author dc:creator
-
- Knudson, Warren
Subjects
dc:subject × 1Identifiers
dc:identifier.*- Identifier
- (UMI)AAI8209598
- OAI identifier oai:identifier
- oai:www.ideals.illinois.edu:2142/70506