Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 255 for “"substrate specificity"”.
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Autoregulatory and structural control of CaMKII substrate specificity
… and synaptic plasticity. CaMKII exhibits broad substrate specificity, targeting numerous substrates with diverse phosphorylation motifs. Binding of the calcium sensor CaM to the autoregulatory domain (ARD) of CaMKII functions to couple kinase activation with calcium signaling. Important sites of …
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Maize β-glucosidase substrate specificity and natural substrates
… the plastid. Numerous glycosides were tested as substrates and K<sub>m</sub>'s and V<sub>m</sub>'s were determined. Various compounds were also tested as inhibitors and K<sub>i</sub>'s and/or K<sub>i</sub>’ 's were estimated. 4- methylumbelliferyl-β-D-glucoside was the best substrate …
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Substrate specificity and functional characterization of sodium/dicarboxylate cotransporters
… helices (TMs) are involved in NaDC1 substrate recognition, with the region of TM 3-4 and the C-terminus required for glutarate while the TM 8-10 region is necessary for adipate transport. Further analysis of these two regions provided evidence that they contained residues important …
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Metabolite biosynthetic enzymes: catalysis, substrate specificity and protein engineering
… superfamily of adenylating enzymes. Here I show substrate-bound structures that identified the enzyme active site and elucidated the mechanistic strategy for pimelate-CoA production. Proper position of reactive groups for the two half-reactions is achieved solely through movements of active site …
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Purification and substrate specificity of new C. roseus enzymes
… TIA biosynthetic enzymes were isolated, and the substrate specificity of the enzymes were known, both natural and novel TIA analogs could be more readily produced on an industrial scale. In this thesis I developed strategies to isolate new C. roseus enzymes and to make novel analogs of the …
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Substrate specificity of [alpha]-proteobacterial N-end rule adaptors
Thesis: Ph. D., Massachusetts Institute of Technology, Department of Biology, 2016.
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Mechanistic evaluation and substrate specificity analysis of a phenylalanine aminomutase
Thesis (Ph. D.)--Michigan State University. Department of Chemistry, 2010
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Mechanism of APC/C activation and substrate specificity in mitosis
… of G1. The APC/C’s catalytic activity and substrate specificity are controlled by its interactions with two coactivators, Cdc20 and Cdh1. In contrast to Cdh1, APC/C activation by Cdc20 during mitosis requires hyper-phosphorylation of APC/C subunits by cyclin-dependent kinase (Cdk) and polo …
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Substrate specificity and properties of heat-stable proteases from Pseudomonas fluorescens
… of oxidized ribonuclease A by proteases of known specificity (trypsin, chymotrypsin, Staphylococcus aureus protease) and the pseudomonal proteases were separated by RP-HPLC. The proteases of known specificity generated a low number of peptides in relatively high concentrations. Proteases of …
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Variation in the Substrate Specificity of Ribulose-1,5-Bisphosphate Carboxylase/oxygenase
… Primary emphasis was on the CO(,2)/O(,2) specificity factor (SF = V(,c)K(,o)/V(,o)K(,c)) which was determined by measuring the rates of carboxylation and oxygenation of RuBp at known concentrations of CO(,2) and O(,2). A sensitive assay procedure was developed for the simultaneous …
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Substrate specificity, active-site residues, and function of the Tsp protease
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 1996.
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Investigating asparagine-linked glycosylation substrate : specificity and effects on protein folding
… of structural modifications for binding-specificity assays and radiolabels for accurate quantification. Access to these substrates allowed us to define the minimum glycosylation consensus sequence for the oligosaccharyltransferase as well as the polyisoprenol specificity of three …
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Probing substrate specificity of RNA repair protein Pnkp/Hen1 from Anabaena variabilis
This is an in vitro study of the substrate specificity of a RNA-repair protein Pnkp/Hen1 from bacteria Anabaena Variabilis. Following the previous discovery of a RNA repair protein—Pnkp/Hen1, which can repair ribotoxin cleaved tRNA in vitro and can add a methyl group at the cleaved site to prevent …
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The Substrate Specificity of Testicular Hyaluronidase and Its Use in Sequencing Chondroitin Sulfates
Testicular hyaluronidase was used to study the distribution of sulfated disaccharide units along chondroitin sulfate chains. The transglycosylation activity of the hyaluronidase was eliminated by use of excess levels of a second enzyme, (beta)-glucuronidase to remove all nonreducing terminal …
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The Active Site Structure and the Substrate Specificity of Cytochrome P-450(cam)
… site structural features in dictating this substrate specificity have been determined with the use of site-directed mutagenesis and specifically modified substrate analogues. In particular, Val-295 forms a complementary fit with the 8,9-gem-dimethyl moiety of camphor and the 10-methyl group …
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Novel peptide-based approaches to study the activity and substrate specificity of protein kinases
… phosphorylation of a tyrosine kinase peptide substrate (Src optimal peptide), the complex was found to bind to the peptide and undergo sensitization via the phosphotyrosine, leading to an increase in luminescence and thus providing a direct measure of kinase activity. To overcome the problem …
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Engineering of the quorum quenching lactonase GcL for altered substrate specificity by rational design
… and these structural changes determine the specificity of the signal. Several strategies that interfere with quorum sensing are naturally used by various organisms. One such strategy is to use enzymes that degrade the AHL signal, known as Quorum Quenching (QQ). Previously characterized QQ …
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