Two-Dimensional Electrode Array Studies of Propagating Epileptiform Neural Activity in the Rat Hippocampal Slice

A technique has been developed in which a planar array of 32 microelectrodes, arranged in a 4 by 8 pattern with 200 μm separation, is used to record from and to stimulate the rat hippocampal slice preparation at multiple sites. Active suppression circuitry is used to prevent device saturation due to large stimulation artifacts. Control of media flow past the tissue is critical to observe signals and to preserve viability. The field potentials recorded are spatially unique and provide a two-dimensional description of the underlying population activity in the various pyramidal cell strata and subpopulations. The application of current source density analysis to the potential data permits accurate determination of peak times, in addition to the estimation of local current sources and sinks. Propagation delays of the compound action potential, first, and second population spikes along CAl calculated using these peak times are not uniform, and may indicate differences and directionality in coupling mechanisms.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Electrical Engineering

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2014

Author dc:creator

• Novak, James Lawrence

• Biology, Neuroscience
• Engineering, Biomedical
• Engineering, Electronics and Electrical

Identifier

(UMI)AAI8823217

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/69401