In Vitro Cultivation of Anaplasma Marginale

Virulent Anaplasma marginale propagated in culture of bovine erythrocytes showed an increasing rate of replication in a sequence of three experiments. The changes in percentage of parasitized erythrocytes (PPE) were monitored by Giemsa staining and the organism was identified by the direct fluorescent antibody (FA) test and transmission electron microscopy. The PPE increased more than 3 times in the first experiment during a period of 14 days. Regression analysis of the data showed that the trend of growth was significantly (P = 0.05) higher after the third passage of the organism in culture. Serologic and ultrastructural studies demonstrated that the in vitro propagated organism was similar to A. marginale isolated from naturally infected bovine blood. Organisms recovered from 13- and 33-day cultures of the first and second experiments were infective for susceptible cattle.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Veterinary Medical Science

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2014

Author dc:creator

• Kessler, Raul Henrique

• Biology, Veterinary Science

Language dc:language

eng

Identifier

(UMI)AAI8017960

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/68231