{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/68231"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/68231","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"In Vitro Cultivation of Anaplasma Marginale","abstract":"Virulent Anaplasma marginale propagated in culture of bovine erythrocytes showed an increasing rate of replication in a sequence of three experiments. The changes in percentage of parasitized erythrocytes (PPE) were monitored by Giemsa staining and the organism was identified by the direct fluorescent antibody (FA) test and transmission electron microscopy. The PPE increased more than 3 times in the first experiment during a period of 14 days. Regression analysis of the data showed that the trend of growth was significantly (P = 0.05) higher after the third passage of the organism in culture. Serologic and ultrastructural studies demonstrated that the in vitro propagated organism was similar to A. marginale isolated from naturally infected bovine blood. Organisms recovered from 13- and 33-day cultures of the first and second experiments were infective for susceptible cattle.","abstract_html":"Virulent Anaplasma marginale propagated in culture of bovine erythrocytes showed an increasing rate of replication in a sequence of three experiments. The changes in percentage of parasitized erythrocytes (PPE) were monitored by Giemsa staining and the organism was identified by the direct fluorescent antibody (FA) test and transmission electron microscopy. The PPE increased more than 3 times in the first experiment during a period of 14 days. Regression analysis of the data showed that the trend of growth was significantly (P = 0.05) higher after the third passage of the organism in culture. Serologic and ultrastructural studies demonstrated that the in vitro propagated organism was similar to A. marginale isolated from naturally infected bovine blood. Organisms recovered from 13- and 33-day cultures of the first and second experiments were infective for susceptible cattle.","abstract_has_math":false,"creators":["Kessler, Raul Henrique"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Veterinary Medical Science","degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2014,"date_issued":"2014-12-14T13:28:22Z","date_published":"2014-12-14T13:28:22Z","updated_at":"2026-07-22T22:25:58Z","subjects":["Biology, Veterinary Science"],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["(UMI)AAI8017960"],"render_values":[{"text":"(UMI)AAI8017960","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/68231","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Kessler, Raul Henrique"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2014-12-14T13:28:22Z","10000-01-01","1980"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Veterinary Medical Science"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology, Veterinary Science"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/68231","(UMI)AAI8017960"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Virulent Anaplasma marginale propagated in culture of bovine erythrocytes showed an increasing rate of replication in a sequence of three experiments. The changes in percentage of parasitized erythrocytes (PPE) were monitored by Giemsa staining and the organism was identified by the direct fluorescent antibody (FA) test and transmission electron microscopy. The PPE increased more than 3 times in the first experiment during a period of 14 days. Regression analysis of the data showed that the trend of growth was significantly (P = 0.05) higher after the third passage of the organism in culture. Serologic and ultrastructural studies demonstrated that the in vitro propagated organism was similar to A. marginale isolated from naturally infected bovine blood. Organisms recovered from 13- and 33-day cultures of the first and second experiments were infective for susceptible cattle.","Ovine erythrocytes infected with the attenuated A. marginale were co-cultured with either normal ovine erythrocytes or normal bovine erythrocytes for 42 days. In both systems, the organism showed an initial period of rapid growth followed by a gradual decrease in PPE and accompanied by cyclic peaks. The presence of bovine erythrocytes in infected ovine erythrocytes resulted in a higher degree of parasitization. Transmission of the organism from infected ovine erythrocytes to normal bovine erythrocytes was demonstrated by use of a two-step FA method which allowed for differentiation between cells and identification of the organism. It is concluded that under in vitro conditions A. marginale is directly transmitted from erythrocyte to erythrocyte without any intermediate cell type.","Made available in DSpace on 2014-12-14T13:28:22Z (GMT). No. of bitstreams: 1 8017960.pdf: 2031687 bytes, checksum: da0a8d96503206839e0c53f7388b7a5f (MD5) Previous issue date: 1980","Embargo set by: Seth Robbins for item 68409 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","72 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1980."]},{"key":"dc:title","label":"Title","values":["In Vitro Cultivation of Anaplasma Marginale"]}]}],"canonical_facts":{"dc:creator":["Kessler, Raul Henrique"],"dc:date":["2014-12-14T13:28:22Z","10000-01-01","1980"],"dc:description":["Virulent Anaplasma marginale propagated in culture of bovine erythrocytes showed an increasing rate of replication in a sequence of three experiments. The changes in percentage of parasitized erythrocytes (PPE) were monitored by Giemsa staining and the organism was identified by the direct fluorescent antibody (FA) test and transmission electron microscopy. The PPE increased more than 3 times in the first experiment during a period of 14 days. Regression analysis of the data showed that the trend of growth was significantly (P = 0.05) higher after the third passage of the organism in culture. Serologic and ultrastructural studies demonstrated that the in vitro propagated organism was similar to A. marginale isolated from naturally infected bovine blood. Organisms recovered from 13- and 33-day cultures of the first and second experiments were infective for susceptible cattle.","Ovine erythrocytes infected with the attenuated A. marginale were co-cultured with either normal ovine erythrocytes or normal bovine erythrocytes for 42 days. In both systems, the organism showed an initial period of rapid growth followed by a gradual decrease in PPE and accompanied by cyclic peaks. The presence of bovine erythrocytes in infected ovine erythrocytes resulted in a higher degree of parasitization. Transmission of the organism from infected ovine erythrocytes to normal bovine erythrocytes was demonstrated by use of a two-step FA method which allowed for differentiation between cells and identification of the organism. It is concluded that under in vitro conditions A. marginale is directly transmitted from erythrocyte to erythrocyte without any intermediate cell type.","Made available in DSpace on 2014-12-14T13:28:22Z (GMT). No. of bitstreams: 1 8017960.pdf: 2031687 bytes, checksum: da0a8d96503206839e0c53f7388b7a5f (MD5) Previous issue date: 1980","Embargo set by: Seth Robbins for item 68409 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","72 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1980."],"dc:identifier":["http://hdl.handle.net/2142/68231","(UMI)AAI8017960"],"dc:language":["eng"],"dc:subject":["Biology, Veterinary Science"],"dc:title":["In Vitro Cultivation of Anaplasma Marginale"],"dc:type":["text"],"thesis:degree_discipline":["Veterinary Medical Science"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:25:58Z"}