Potassium and Sodium Movements Related to Growth State and Malignant Transformation in Cultured Chicken Embryo Cells

Aspects of sodium-potassium pump regulation were examined in growing normal, density-inhibited, and Rous sarcoma virus-transformed (RSV-transformed) chicken embryo fibroblasts and following serum stimulation of quiescent, density-inhibited chicken embryo fibroblasts. Active potassium transport and the associated specific ouabain binding were shown to be dependent on the cellular growth state but unaffected by malignant transformation; potassium influx and ouabain binding values in growing normal and transformed chicken embryo fibroblasts were 1.5 to 1.8-fold greater than in density-inhibited cells. Growing normal and density-inhibited cells were found to have similar potassium contents and RSV-transformed cells a 1.4-fold higher potassium content, as determined by both flame photometry and and isotopic equilibration. Based on the percentage of potassium pool exiting the cell, potassium efflux was found to be 1.3 to 1.5-fold higher in growing normal than RSV-transformed or density-inhibited cells; efflux based on the absolute number of potassium ions is similar in growing normal and transformed cells because of the larger potassium pool in transformed cells.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Microbiology

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2014

Author dc:creator

• Johnson, Marcia Anne

• Biology, Microbiology

Language dc:language

eng

Identifier

(UMI)AAI8017955

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/68090