Investigation of Ribosome Structure and Ribosome-Transfer Rna Interaction Using T4 Rna Ligase

T4 RNA ligase can be used to alter the sequence of E. coli ribosomal RNAs in the intact polyribosome, ribosome and subunits. This enzyme will catalyze the addition of a 3',5'-bisphosphate onto the 3' terminus of rRNAs resulting the rRNA molecules one nucleotide longer with a 3' phosphate. The RNA ligase catalyzed addition of radioactively labeled cytidine 3',5'-bisphosphate has been used to probe the availability of the 3' ends of rRNAs in the ribosome under varying conditions.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Biochemistry

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2014

Author dc:creator

• Rose, Samuel Jahile, Iii

• Chemistry, Biochemistry

Language dc:language

eng

Identifier

(UMI)AAI8127682

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/67413