Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 38 for “"Transfer RNA"”.
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Organization of Transfer-Rna Genes in Drosophila
Made available in DSpace on 2014-12-14T04:27:45Z (GMT). No. of bitstreams: 1 7913439.pdf: 5450960 bytes, checksum: 5b64a66820e1e9269a6c4f02698f5f9f (MD5) Previous issue date: 1978
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Nucleotides in yeast phenylanyl - transfer-RNA required for the specific recognition by its cognate yeast phenylalanyl - transfer-RNA synthetase
A method for synthesizing yeast tRNA$\sp{\rm Phe}$ mutants having nucleotide substitutions at any desired position in the tRNA has been developed. Using synthetic DNA oligonucleotides, recombinant plasmids were constructed with a T7 promoter directly upstream of the tRNA gene and a BstN 1 …
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Characterization of Transfer RNA Associated with Plasma Membranes
RNA extracted from purified rat liver plasma membranes was found to contain transfer RNA. Amino acid acceptor activity was detected for arginine and lysine, a confirmation of previous reports. In addition, acceptor activity was also detected for tryptophan and proline. Isoaccepting tRNA species for …
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Modification of Transfer Rna With T4 Rna Ligase
T4 RNA ligase can be used to alter the sequence of transfer RNAs. This enzyme will catalyze the addition of a 3',5'-bisphosphate onto the 3' terminus of a tRNA resulting in a tRNA molecule one nucleotide longer with a 3' phosphate. Under appropriate conditions the reaction is quantitative and, if …
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A functional role for transfer RNA-derived microRNAs in human B cells
… organisms express a diverse repertoire of small RNAs. Genetic and biochemical investigation of these molecules has demonstrated that many small RNAs, most notably the microRNA subclass, possess the capacity to influence the expression of other genes, adding substantial complexity to our …
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A cluster of two serine transfer RNA genes from Clostridium acetobutylicum P262
A cloning system, using metronidazole as a screening tool and E. coli FI9 as a selection host, was previously established to clone C. acetobutylicum P262 electron transport genes which may play a role in solvent metabolism in this bacterium. In theory, metronidazole would be reduced under anaerobic …
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Investigation of Ribosome Structure and Ribosome-Transfer Rna Interaction Using T4 Rna Ligase
T4 RNA ligase can be used to alter the sequence of E. coli ribosomal RNAs in the intact polyribosome, ribosome and subunits. This enzyme will catalyze the addition of a 3',5'-bisphosphate onto the 3' terminus of rRNAs resulting the rRNA molecules one nucleotide longer with a 3' phosphate. The RNA …
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Studies of Initiation of Protein Synthesis and Activating Enzyme: Transfer-Rna Complex Formation
Made available in DSpace on 2014-12-10T23:24:31Z (GMT). No. of bitstreams: 1 7405621.pdf: 5968154 bytes, checksum: 5ea803a72d01d13b85e69c2cb869d119 (MD5) Previous issue date: 1973
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Evolution and dynamic behavior of transfer RNA in the first two steps of translation
… a key component of the cellular machinery is transfer RNA (tRNA). This small nucleic acid is crucial to the maintenance of the genetic code because it discriminately binds the messenger RNA codon at the ribosome and adds the cognate amino acid to the growing polypeptide chain. The role of tRNA …
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FLUORESCENCE SPECTROSCOPY AND PRE-STEADY STATE KINETIC STUDIES ON E. COLI METHIONYL TRANSFER RNA SYNTHETASE
… in <italic>Escherichia coli</italic> methionyl tRNA synthetase (MetRS). In this thesis we utilize fluorescence spectroscopy and pre-steady state kinetics to unveil some features of MetRS catalysis that were previously unclear.
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Transfer rna dynamics and transfer-messenger rna accommodation in bacterial ribosomes at the single-molecule level
… (PIFE), fluorescence resonance energy transfer (FRET), and several biochemical tools were applied to study transfer RNA (tRNA) dynamics and transfer-messenger RNA (tmRNA) accommodation inside ribosomes. In the first project of this thesis work, structural characterization of the …
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The Cellular and Molecular Dynamics of the Queuosine Modification in Transfer RNA: Definition, Modulation, Deficiencies and Effect of the Queuosine Modification System
… modification in the wobble position of tRNA<sup>asn</sup>, tRN<sup>asp</sup>, tRNA<sup>his</sup>, and tRNA<sup>tyr</sup> is associated with a decrease in cellular growth rate, an increase in the ability to withstand environmental stress, and differentiation of pleuripotent cells into …
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Studies of the Mechanism of Biosynthesis of N(6)-(delta(2)-Isopentenyl)-2-Thiomethyladenosine in E. Coli Tyrosine-Specific Suppressor Transfer-Rna
Made available in DSpace on 2014-12-14T04:27:31Z (GMT). No. of bitstreams: 1 7524340.pdf: 3686016 bytes, checksum: da516196234e92907a86933e2bc64b83 (MD5) Previous issue date: 1975
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The Role of Initiation Factor Dynamics in Translation Initiation
… single-molecule fluorescence resonance energy transfer (smFRET) as a primary tool for investigating how the dynamics of IF1 and IF3 regulate the accuracy with which the translational machinery selects an initiator transfer RNA (tRNA) and the correct messenger RNA (mRNA) start codon during the …
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Processes involved in protein translation
… use the same basic components—such as the transfer RNA (tRNA); aminoacyl-tRNA synthases; and the ribosome and its various elongation, initiation, and release factors—to carry out the translation process. It is the latter group, ribosomes and associated proteins, that physically make the …
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Biochemical Analysis of the Molecular Factors of Leucyl-Trna Synthetase That Ensure Aminoacylation Fidelity
The tRNA synthetases catalyze aminoacylation of transfer RNA (tRNA) with specific amino acids. Accurate aminoacylation is critical to the fidelity of protein synthesis. Leucyl-tRNA synthetase (LeuRS) can misactivate a broad scope of non-leucine amino acids. In these cases, mischarged tRNA products …
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Experimental and Computational Dynamics of an Aminoacyl-tRNA Synthetase System
… upon correct recognition and aminoacylation of transfer RNA (tRNA) by their cognate aminoacyl-tRNA synthetases (AARSs). Methionine is the amino acid that acts as the start codon for every protein and can also be found within a protein message. Methionyl-tRNA synthetase (MetRS) is responsible for …
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