Modification of Transfer Rna With T4 Rna Ligase

T4 RNA ligase can be used to alter the sequence of transfer RNAs. This enzyme will catalyze the addition of a 3',5'-bisphosphate onto the 3' terminus of a tRNA resulting in a tRNA molecule one nucleotide longer with a 3' phosphate. Under appropriate conditions the reaction is quantitative and, if high specific radioactivity bisphosphates are used, it provides an efficient means for in vitro labeling of tRNA. Although the 3' terminal hydroxyl is a good acceptor, the 5' terminal phosphate in most tRNAs is not an effective donor in the T4 RNA ligase reaction. This poor reactivity is due to the secondary structure at the 5' terminus. If E. coli tRNA('fMet) is used, the 5' phosphate is reactive and the major product with T4 RNA ligase is the cyclic tRNA.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Biochemistry

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2014

Author dc:creator

• Bruce, Allen Gregory

• Chemistry, Biochemistry

Language dc:language

eng

Identifier

(UMI)AAI8127555

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/67412