An Analysis of the Collagen Synthesized by Chondrocyte Cultures Derived From the Chick Embryo Tibiotarsus

Chondrocyte cell cultures originating from the histologically distinct zones of the tibiotarsus synthesize chondroitin sulfate and type II collagen in primary and secondary cultures. The collagen is secreted into two extracellular collagen pools, the medium (CM) and matrix (Ma) fractions. The CM-collagen fraction contains procollagen and the intermediates in its metabolic maturation to {(alpha)1(II)}(,3) collagen. The Ma-collagen fraction contains mostly the pN-collagen and fully processed collagen. Small amounts of other bands that have a mobility slightly slower than the (alpha)1 chains are seen in this fraction after limited pepsin digestion. Under the cell culture conditions examined, the kinetics of collagen accumulation was found to be very similar for the cells derived from each of the distinct zones. The kinetic experiments also showed type II collagen to be synthesized on an equal weight basis with chondroitin sulfate.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Biochemistry

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2014

Author dc:creator

• Schmid, Thomas Michael

• Chemistry, Biochemistry

Language dc:language

eng

Identifier

(UMI)AAI8108654

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/67407