The Synthesis of Oligodeoxyribonucleotides With Rna Ligase

RNA ligase, isolated from bacteriophage T4-infected Escherichia coli catalyzes the ATP-dependent formation of a 3' (--->) 5'-phosphodiester bond between one oligonucleotide with a 3'-hydroxyl group (the acceptor molecule) and another with a 5'-phosphate (the donor molecule). The enzyme does not require the alignment of the donor and acceptor molecules onto a complementary template strand, and the reaction can yield an unique product. For these reasons, RNA ligase has proved useful for the synthesis of oligoribonucleotides of defined sequence. This work involves the extension of the RNA ligase reaction to DNA substrates and, in particular, the use of the single nucleotide addition reaction of RNA ligase with DNA acceptors.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Biochemistry

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2014

Author dc:creator

• Hinton, Deborah Meetze

• Chemistry, Biochemistry

Language dc:language

eng

Identifier

(UMI)AAI8026520

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/67400