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Showing 1 to 17 of 17 for “"RNA ligase"”.
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The Synthesis of Oligodeoxyribonucleotides With Rna Ligase
RNA ligase, isolated from bacteriophage T4-infected Escherichia coli catalyzes the ATP-dependent formation of a 3' (--->) 5'-phosphodiester bond between one oligonucleotide with a 3'-hydroxyl group (the acceptor molecule) and another with a 5'-phosphate (the donor molecule). The enzyme does not …
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Intermolecular Joining of Dna Oligonucleotides With Rna Ligase
Made available in DSpace on 2014-12-14T04:27:48Z (GMT). No. of bitstreams: 1 8009101.pdf: 6452301 bytes, checksum: 2b7980a57152a1c82231d5c8a4574206 (MD5) Previous issue date: 1979
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Modification of Transfer Rna With T4 Rna Ligase
T4 RNA ligase can be used to alter the sequence of transfer RNAs. This enzyme will catalyze the addition of a 3',5'-bisphosphate onto the 3' terminus of a tRNA resulting in a tRNA molecule one nucleotide longer with a 3' phosphate. Under appropriate conditions the reaction is quantitative and, if …
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The Phosphatase Activities of T4 Polynucleotide Kinase (Rna Ligase, Splicing)
… the 2':3'-cyclic phosphate in the mechanism of tRNA splicing in wheat germ and yeast prompted a closer examination of the 3'-phosphatase activity of T4 polynucleotide kinase. This resulted in the discovery of not only a 2':3'-cyclic phosphatase activity but also a 2'-phosphatase, in addition to …
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Nucleotide recognition and metal ion requirements of an RNA ligase ribozyme
The class I ligase, a ribozyme previously isolated from random sequence, catalyzes a reaction similar to RNA polymerization, positioning its 5'-nucleotide via a Watson-Crick base pair, forming a 3',5'-phosphodiester bond between its 5'-nucleotide and the substrate, and releasing pyrophosphate. The …
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Investigation of Ribosome Structure and Ribosome-Transfer Rna Interaction Using T4 Rna Ligase
T4 RNA ligase can be used to alter the sequence of E. coli ribosomal RNAs in the intact polyribosome, ribosome and subunits. This enzyme will catalyze the addition of a 3',5'-bisphosphate onto the 3' terminus of rRNAs resulting the rRNA molecules one nucleotide longer with a 3' phosphate. The RNA …
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Biotin and Fluorescent Labeling of Ribonucleic Acids Using T4 Rna Ligase (Rna Modification, Rna-Dna Hybridization)
… were synthesized and used as substrates with T4 RNA ligase. In the absence of ATP, the non-adenylyl portion of these substrates is transferred to the 3'-hydroxyl of an RNA molecule (acceptor) to form a phosphodiester bond and the AMP portion is released. E. coli and D. melanogaster 5S RNA, yeast …
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Substitution of the Anticodon Loop of Yeast Tyrosine Transfer Ribonucleic Acid (Rna)
… residues 33-35 in the anticodon loop of yeast tRNA('Tyr) with any desired oligonucleotide have been developed. Half-molecules of tRNA('Tyr) from partial digestions with ribonuclease A or T(,1) were paired to create a gap in the anticodon loop. An oligonucleotide was inserted into the gap using …
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Specific Bacteriophage Coat Protein-Rna Interactions
… operator variants have been synthesized by using RNA ligase and transcription of synthetic DNA by T7 RNA polymerase in order to examine the roles of the helical region and the bulged A residue in the specific RNA-R17 coat protein interaction. The affinity between coat protein and each variant was …
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Characterization and evolution of artificial RNA ligases
… focused on the characterization of an artificial RNA ligase previously selected from a library of polypeptide variants based on a non-catalytic protein scaffold. The selection employed mRNA display, a technique to isolate de novo enzymes in vitro from large libraries of 1013 protein variants. The …
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[RNA polymerase ribozymes]
The RNA World is a hypothetical ancient evolutionary era during which RNA was both genome and catalyst. During that time, RNA was the only kind of enzyme yet in existence, and one of its chief duties was the replication of RNA. This scenario presupposes that among all possible RNA sequences, there …
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Discovery and characterization of novel RNA repair systems
RNA is one of the major macromolecules essential for all known forms of life. RNA in the cell is subject to many types of damage as DNA, and the ubiquitous RNA degradation by surveillance machinery is an important way to respond RNA damage. However, RNA repair is an alternative way for cells to …
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The origin and evolution of the ribosome
… elements of the ribosome, namely ribosomal RNA (rRNA) and ribosomal proteins (r-proteins) provided a deep and the most comprehensive insight possible yet into the origins and evolution of the ribosome. Using phylogenetic methods that reconstruct the evolutionary history of complex RNA and …
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Biochemical and structural studies of RNA damage and repair in bacteria
… endonucleases which typically target RNA molecules that are necessary for translation mainly, tRNAs and rRNAs which can result in their loss of function. In order to respond to RNA damage, some organisms have developed RNA repair systems which can heal and seal the cleaved RNA molecule …
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Entwicklung einer Synthesestrategie aminoacylierter Dimerfragmente der tRNA mittels Festphasensynthese
Für die Synthese misacylierter tRNA’s existiert ein Verfahren, welches von einer 3‘-seitig um zwei Nukleotide verkürzten tRNA ausgeht, die mittels T4 RNA Ligase mit einem zuvor über chemische Methoden aminoacylierten Dimerfragment (pdCpA-naa, naa: nicht natürliche Aminosäure) zu einer kompletten, …
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Evaluation and adaptation of molecular approaches for detection and characterization of viruses of the respiratory tract
… but ssDNA could not be amplified at all. Alternative protocols were adapted for ligating ssDNA and dsDNA to construct large concatemers for efficient phi29-amplification. Phi29-amplification of ssDNA was found to increase >10-fold when it was ligated using T4 RNA ligase in the presence of a …
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Global transcriptional, post-transcriptional, and translational regulation during soybean seed and seedling development
… the gene regulatory networks, high-throughput RNA sequencing (RNA-Seq) was carried out using cotyledons from seven developmental stages of soybean seedlings. A total of 154 genes demonstrated higher level of expression exclusively in the early seedling stages. About 25% of those genes with …