A spectroscopic smartphone biosensor for use in point-of-care diagnostic applications

This thesis serves to introduce a new point-of-care testing device capable of providing spectrometric data for solid and liquid phase samples. A custom cradle was developed to maintain optical alignment allowing for the diffraction, and therefore spectrometric analysis, of light transmitted either directly through a sample or via an intermediary optical biosensor. Demonstration of the instrument is provided with both an enzyme-linked immunosorbent assay (ELISA) and a photonic-crystal (PC)-based investigation of biomolecular adsorption. ELISA analysis is completed on both human interleukin-6, an important diagnostic cancer biomarker, and Ara h 1, one of the principle peanut proteins responsible for allergic reactions. PC-based sensing was completed with a Protein A-immunoglobulin G interaction with a .009 nm accuracy. All of these demonstrations were completed at physiologically-relevant concentrations, and as such, serve as proof-of-concept for two separate modalities of a smartphone-based spectrometric optical biosensor. In the future, such a sensor has the potential to provide a low-cost, point-of-care diagnostic device that is inherently robust, user-friendly, cloud-connected, and suited for use in remote sensing applications.

Name thesis:degree_name

M.S.

Level thesis:degree_level

Thesis

Discipline thesis:degree_discipline

Bioengineering

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2014

Author dc:creator

• Long, Kenneth

Contributors dc:contributor

• Cunningham, Brian T.

• Smartphone Biosensing
• Photonic Crystal
• Enzyme-Linked Immunosorbent Assay
• Mobile Biosensing
• Point-of-Care Diagnostics

Statement dc:rights

• Copyright 2014 Kenneth D. Long

Language dc:language

en

Handle dc:identifier

http://hdl.handle.net/2142/49844

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/49844