RNA-SEQ driven analysis of seasonal gene expression variation in miscanthus �� giganteus

Miscanthus × giganteus is a C4 grass that has generated a large amount of interest as a potential biofuel crop due to its high level of biomass production, its perenniality, and its sterility. In this study, two separate M. × giganteus RNA-Seq datasets were generated to help explore the characteristics of M. × giganteus at the level of gene expression: a ten-tissue dataset suitable for examining genes with tissue-preferred expression, and a twenty-four sample dataset for examining the changes in gene expression that occur over the growing season. Aided by these datasets, aspects and potential mediators of M. × giganteus’ seasonal developmental cycle and changes in the utilization, storage, and long-distance mobilization and remobilization of the essential nutrient nitrogen have been studied. These RNA-Seq datasets have been verified with RT-qPCR and compared to amino acid and elemental concentration profiles; as a result, many seasonal changes in gene expression corresponding to the growth and development of M. × giganteus have been documented in order to better define the traits that make this crop such an outstanding biofuel candidate.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Crop Sciences

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2014

Author dc:creator

• Barling, Adam R.

Contributors dc:contributor

• Moose, Stephen P.
• Hudson, Matthew E.
• Brown, Patrick J.
• Bernacchi, Carl J.

• Ribonucleic Acid Sequencing (RNA-Seq)
• Miscanthus
• Biofuel

Statement dc:rights

• Copyright 2013 Adam Robert Barling

Language dc:language

en

Handle dc:identifier

http://hdl.handle.net/2142/46926

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/46926