{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/46926"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/46926","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"RNA-SEQ driven analysis of seasonal gene expression variation in miscanthus �� giganteus","abstract":"Miscanthus × giganteus is a C4 grass that has generated a large amount of interest as a potential biofuel crop due to its high level of biomass production, its perenniality, and its sterility. In this study, two separate M. × giganteus RNA-Seq datasets were generated to help explore the characteristics of M. × giganteus at the level of gene expression: a ten-tissue dataset suitable for examining genes with tissue-preferred expression, and a twenty-four sample dataset for examining the changes in gene expression that occur over the growing season. Aided by these datasets, aspects and potential mediators of M. × giganteus’ seasonal developmental cycle and changes in the utilization, storage, and long-distance mobilization and remobilization of the essential nutrient nitrogen have been studied. These RNA-Seq datasets have been verified with RT-qPCR and compared to amino acid and elemental concentration profiles; as a result, many seasonal changes in gene expression corresponding to the growth and development of M. × giganteus have been documented in order to better define the traits that make this crop such an outstanding biofuel candidate.","abstract_html":"Miscanthus × giganteus is a C4 grass that has generated a large amount of interest as a potential biofuel crop due to its high level of biomass production, its perenniality, and its sterility. In this study, two separate M. × giganteus RNA-Seq datasets were generated to help explore the characteristics of M. × giganteus at the level of gene expression: a ten-tissue dataset suitable for examining genes with tissue-preferred expression, and a twenty-four sample dataset for examining the changes in gene expression that occur over the growing season. Aided by these datasets, aspects and potential mediators of M. × giganteus’ seasonal developmental cycle and changes in the utilization, storage, and long-distance mobilization and remobilization of the essential nutrient nitrogen have been studied. These RNA-Seq datasets have been verified with RT-qPCR and compared to amino acid and elemental concentration profiles; as a result, many seasonal changes in gene expression corresponding to the growth and development of M. × giganteus have been documented in order to better define the traits that make this crop such an outstanding biofuel candidate.","abstract_has_math":false,"creators":["Barling, Adam R."],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Crop Sciences","degree_department":null,"school":null,"contributors":["Moose, Stephen P.","Hudson, Matthew E.","Brown, Patrick J.","Bernacchi, Carl J."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2014,"date_issued":"2014-01-16T18:26:33Z","date_published":"2014-01-16T18:26:33Z","updated_at":"2026-07-22T22:25:38Z","subjects":["Ribonucleic Acid Sequencing (RNA-Seq)","Miscanthus","Biofuel"],"languages":["en"],"rights":["Copyright 2013 Adam Robert Barling"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/2142/46926","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Moose, Stephen P.","Hudson, Matthew E.","Brown, Patrick J.","Bernacchi, Carl J."]},{"key":"dc:creator","label":"Author","values":["Barling, Adam R."]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2014-01-16T18:26:33Z","2016-01-16T11:00:41Z","2013-12"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Crop Sciences"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Ribonucleic Acid Sequencing (RNA-Seq)","Miscanthus","Biofuel"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2013 Adam Robert Barling"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/46926"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Miscanthus × giganteus is a C4 grass that has generated a large amount of interest as a potential biofuel crop due to its high level of biomass production, its perenniality, and its sterility. 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These RNA-Seq datasets have been verified with RT-qPCR and compared to amino acid and elemental concentration profiles; as a result, many seasonal changes in gene expression corresponding to the growth and development of M. × giganteus have been documented in order to better define the traits that make this crop such an outstanding biofuel candidate.","Item withdrawn by Laura Spradlin (lspradl2@illinois.edu) on 2013-11-12T14:41:23Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 2 Barling_Adam.docx: 5798608 bytes, checksum: 4fe2a4fbc858000cc536fc5b70c94af6 (MD5) Barling_Adam.pdf: 4335052 bytes, checksum: d8edab60a291d990915592d6dd7ccd51 (MD5)","Made available in DSpace on 2014-01-16T18:26:33Z (GMT). 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