University of Illinois - Urbana-Champaign
Biophysical Applications of Single Molecule Fluorescence Localization
Abstract
dc:descriptionFluorescence single molecule localization has become a powerful tool for investigating problems in biological physics. However, there are numerous limitations on the types of measurements that can be made with typical instrument designs based on objective-type TIR and wide field CCD imaging. We present several novel applications of single molecule localization to both proteomics and genomics, including a method for improving the resolving capacity by more than an order of magnitude (Single Molecule High Resolution Imaging with Photo-bleaching, or SHRImP), a method for extending wide field single-molecule localization to three dimensions (Three Dimensional Imaging with One Nanometer Accuracy, or 3IONA), and applications of these techniques to problems in genomics and molecular motors.
Degree
thesis:*- Name thesis:degree_name
- Ph.D.
- Level thesis:degree_level
- Dissertation
- Discipline thesis:degree_discipline
- Physics
- Year dc:date
- 2012
Author and committee
dc:creator, dc:contributor.*- Author dc:creator
-
- Gordon, Matthew Paul
- Contributors dc:contributor
-
- Selvin, Paul R.
Subjects
dc:subject × 3Rights
dc:rights- Statement dc:rights
-
- ©2006 Matthew Paul Gordon
- Language dc:language
- en
Identifiers
dc:identifier.*- Identifier
- 5639998
- OAI identifier oai:identifier
- oai:www.ideals.illinois.edu:2142/34737